Next Article in Journal
A 2.45 GHz High Efficiency CMOS RF Energy Harvester with Adaptive Path Control
Next Article in Special Issue
Multi-Channel Transfer Learning of Chest X-ray Images for Screening of COVID-19
Previous Article in Journal
A Modified Model Reference Adaptive Controller (M-MRAC) Using an Updated MIT-Rule for the Altitude of a UAV
Article

Intercellular Bioimaging and Biodistribution of Gold Nanoparticle-Loaded Macrophages for Targeted Drug Delivery

1
Department of Biomedical Engineering, Beckman Laser Institute Korea, College of Medicine, Dankook University, 119, Dandae-ro, Dongnam-gu, Cheonan, Chungnam 31116, Korea
2
Department of Biomedical Sciences, Hallym University College of Medicine, Chuncheon 24252, Korea
3
Department of Oral & Maxillofacial Surgery, Dentistry, Hallym University College of Medicine, Anyang 14068, Korea
4
Tomocube Inc., 155, Sinseong-ro, Yuseong-gu, Daejeon 34109, Korea
5
Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Korea
6
Beckman Laser Institute and Medical Clinic, University of California, Irvine 1002 Health Sciences Rd, Irvine, CA 92617, USA
7
Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Dongtan Sacred Heart Hospital, 7, Keunjaebong-gil, Hwaseong-si, Gyeonggi-do 18450, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally in this study.
Electronics 2020, 9(7), 1105; https://doi.org/10.3390/electronics9071105
Received: 27 May 2020 / Revised: 20 June 2020 / Accepted: 2 July 2020 / Published: 7 July 2020
(This article belongs to the Special Issue Optical Sensing for Biomedical Applications)
In order to effectively apply nanoparticles to clinical use, macrophages have been used as vehicles to deliver genes, drugs or nanomaterials into tumors. In this study, the effectiveness of macrophage as a drug delivery system was validated by biodistribution imaging modalities at intercellular and ex vivo levels. We focused on biodistribution imaging, namely, the characterization of the gold nanoparticle-loaded macrophages using intracellular holotomography and target delivery efficiency analysis using ex vivo fluorescence imaging techniques. In more detail, gold nanoparticles (AuNPs) were prepared with trisodium citrate method and loaded into macrophage cells (RAW 264.7). First, AuNPs loading into macrophages was confirmed using the conventional ultraviolet-visible (UV-VIS) spectroscopy and inductively coupled plasma-mass spectrometry (ICP-MS). Then, the holotomographic imaging was employed to characterize the intracellular biodistribution of the AuNPs-loaded macrophages. The efficacy of target delivery of the well AuNPs uptake macrophages was studied in a mouse model, established via lipopolysaccharide (LPS)-induced inflammation. The fluorescent images and the ex vivo ICP-MS evaluated the delivery efficiency of the AuNPs-loaded macrophages. Results revealed that the holotomographic imaging techniques can be promising modalities to understand intracellular biodistribution and ex vivo fluorescence imaging can be useful to validate the target delivery efficacy of the AuNPs-loaded macrophages. View Full-Text
Keywords: gold nanoparticle; macrophage; biodistribution; holotomography; drug delivery System gold nanoparticle; macrophage; biodistribution; holotomography; drug delivery System
Show Figures

Figure 1

MDPI and ACS Style

Kim, S.; Kang, S.H.; Byun, S.H.; Kim, H.-J.; Park, I.-K.; Hirschberg, H.; Hong, S.J. Intercellular Bioimaging and Biodistribution of Gold Nanoparticle-Loaded Macrophages for Targeted Drug Delivery. Electronics 2020, 9, 1105. https://doi.org/10.3390/electronics9071105

AMA Style

Kim S, Kang SH, Byun SH, Kim H-J, Park I-K, Hirschberg H, Hong SJ. Intercellular Bioimaging and Biodistribution of Gold Nanoparticle-Loaded Macrophages for Targeted Drug Delivery. Electronics. 2020; 9(7):1105. https://doi.org/10.3390/electronics9071105

Chicago/Turabian Style

Kim, Sehwan, Sung H. Kang, Soo H. Byun, Hye-Jin Kim, In-Kyu Park, Henry Hirschberg, and Seok J. Hong. 2020. "Intercellular Bioimaging and Biodistribution of Gold Nanoparticle-Loaded Macrophages for Targeted Drug Delivery" Electronics 9, no. 7: 1105. https://doi.org/10.3390/electronics9071105

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop