High-Frequency and High-Voltage Asymmetric Bipolar Pulse Generator for Electroporation Based Technologies and Therapies

Round 1

Reviewer 1 Report

Authors present quite interesting work in this manuscript. One minor comment would like to provide here for authors' reference is that can authors provide simple plot to explain the design of experiment (DOE) in this work, specifically in the section 2.2.1？How the actually "test" DOE in your DOE and how does that setting mounted in mice (e.g. group i to ix)？That would be helpful for this referee and potential readers to get further insight into what would be the experimental groups in this manuscript. 

Author Response

Thank you very much for Your comments and encouraging opinion about our work.  As recommended, we added Figure 3 and Table 1 to further explain the design of the animal experiments and experimental groups in the section 2.2.1. of our manuscript.

Reviewer 2 Report

Authors propose a high frequency (minimum pulse width 200ns), high voltage (up to 4kV) asymmetric bipolar pulse generator for electroporation based therapies, in this case electrochemoterapeutic treatment for cancer reduction. The paper is well-written, an exhaustive introduction precedes a description of the proposed system and in-vivo tests. Results under equivalent load and measurements in-vivo are reported and compared with other treatments. No comparison with prior art are reported because the lack of previous studies which use the same approach.

Generally speaking, the study described in the paper is very interesting and easily to follow, also for a neophyte for this topic. Only few typos are present in the text.

From the point of view of the circuital aspect, the proposed system has a limited novelty as compared with pulse generators exploited in other applications, such as piezoelectric actuators, membrane excitation and so on. However, achieved performances have been allowed thanks to an accurate choice of the components and results are shown in a satisfacient mode.

My only dubt is if electronics is the most suitable magazine of mdpi where submit this work, since it is not well-defined what the authors want to propose, the pulse generator or the method, and thus the results of the application of the pulse generatore itself.

Author Response

Thank you very much for Your comments and encouraging opinion about our work. We re-read the manuscript and removed the typos and grammar errors. We believe that our topic is in the scope of the Journal, however, this decision is to be made by the Editors of the Journal.