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Open AccessArticle

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm

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Department of Mathematics and Physics E. de Giorgi, University of Salento, 73100 Via per Arnesano, Lecce, Italy
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Institute of Nanotechnology, CNR NANOTEC, 73100 Via per Monteroni, Lecce, Italy
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Scuola Superiore ISUFI (Istituto Superiore Universitario di Formazione Interdisciplinare), University of Salento, 73100 Via per Arnesano, Lecce, Italy
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Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Via per Monteroni, Lecce, Italy
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Center for Biomolecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, Via Barsanti, I-73010 Arnesano, Lecce, Italy
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Department of Biosciences, Biotechnologies & Biopharmaceutics, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy
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Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini, 10095 Grugliasco, Torino, Italy
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Department of Veterinary Medicine, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy
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Author to whom correspondence should be addressed.
Biology 2020, 9(2), 22; https://doi.org/10.3390/biology9020022
Received: 12 December 2019 / Revised: 23 January 2020 / Accepted: 25 January 2020 / Published: 27 January 2020
(This article belongs to the Section Cell Biology)
Conventional sperm selection techniques used in ARTs rely on centrifugation steps. To date, the different studies reported on the effects of centrifugation on stallion sperm motility provided contrasting results and do not include effects on mitochondrial functionality and different oxidative parameters. The effects of different centrifugation protocols (300× g for 5′, 300× g for 10′, 1500× g for 5′ and 1500× g for 10′ vs. no centrifugation) on motility and oxidative status in cryopreserved stallion sperm, were analyzed. After centrifugation, almost all motility parameters were significantly altered, as observed by computer-assisted sperm analysis. A polarographic assay of oxygen consumption showed a progressive decrease in mitochondria respiration from the gentlest to the strongest protocol. By laser scanning confocal microscopy, significant reduction of mitochondrial membrane potential, at any tested protocol, and time-dependent effects, at the same centrifugal force, were found. Increased DNA fragmentation index at any tested protocol and time-dependent effects at the same centrifugal force were found, whereas increased protein carbonylation was observed only at the strongest centrifugal force. These results provide more comprehensive understandings on centrifugation-induced effects on cryopreserved stallion sperm and suggest that, even at a weak force for a short time, centrifugation impairs different aspects of equine sperm metabolism and functionality. View Full-Text
Keywords: sperm motility; mitochondria; oxidative stress; DNA fragmentation; assisted reproductive technology sperm motility; mitochondria; oxidative stress; DNA fragmentation; assisted reproductive technology
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MDPI and ACS Style

Marzano, G.; Moscatelli, N.; Di Giacomo, M.; Martino, N.A.; Lacalandra, G.M.; Dell’Aquila, M.E.; Maruccio, G.; Primiceri, E.; Chiriacò, M.S.; Zara, V.; Ferramosca, A. Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology 2020, 9, 22. https://doi.org/10.3390/biology9020022

AMA Style

Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell’Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology. 2020; 9(2):22. https://doi.org/10.3390/biology9020022

Chicago/Turabian Style

Marzano, Giuseppina; Moscatelli, Natalina; Di Giacomo, Mariangela; Martino, Nicola A.; Lacalandra, Giovanni M.; Dell’Aquila, Maria E.; Maruccio, Giuseppe; Primiceri, Elisabetta; Chiriacò, Maria S.; Zara, Vincenzo; Ferramosca, Alessandra. 2020. "Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm" Biology 9, no. 2: 22. https://doi.org/10.3390/biology9020022

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