Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1α2
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Animals
2.3. Liver and Spleen Index
2.4. Hematological Analyses
2.5. Liver Enzyme Quantification
2.6. Enzyme Linked Immunosorbent Assay (ELISA)
2.7. Liver Histological, Immunohistochemistry and Immunofluorescence Analysis
2.8. Bioinformatics Analysis of miRNA Targets
2.9. Dual-Luciferase Reporter Assay
2.10. Fluorescence In Situ Hybridization (FISH)
2.11. RNA Extraction and mRNA/miRNA Quantification
2.12. Establishment of Schistosome-Induced Liver Fibrosis Mice Model
2.13. Statistical Analysis
3. Results
3.1. Sja-Let-7 Attenuated Progression of CCL4-Induced LF in Mice
3.2. Sja-Let-7 Diminished Pro-Fibrotic and Pro-Inflammatory Cytokines in CCL4-Induced LF
3.3. Sja-Let-7 Regulates LF via Targeting Col1α2
3.4. Sja-Let-7 Regulates LF via the TGF-β/Smad Pathway
3.5. Sja-Let-7 Reduces Schistosome-Induced LF
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhong, H.; Dong, B.; Zhu, D.; Li, H.; Lu, K.; Fu, Z.; Liu, J.; Jin, Y. Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1α2. Biology 2023, 12, 1465. https://doi.org/10.3390/biology12121465
Zhong H, Dong B, Zhu D, Li H, Lu K, Fu Z, Liu J, Jin Y. Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1α2. Biology. 2023; 12(12):1465. https://doi.org/10.3390/biology12121465
Chicago/Turabian StyleZhong, Haoran, Bowen Dong, Danlin Zhu, Hao Li, Ke Lu, Zhiqiang Fu, Jinming Liu, and Yamei Jin. 2023. "Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1α2" Biology 12, no. 12: 1465. https://doi.org/10.3390/biology12121465