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Article

Dysregulation in the Expression of Platelet Surface Receptors in Acute Coronary Syndrome Patients—Emphasis on P2Y12

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Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland
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Biohazard Prevention Centre, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland
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Department of Pharmacology and Toxicology, Medical University of Lodz, 90-752 Lodz, Poland
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Department of Interventional Cardiology, Medical University of Lodz, 91-213 Lodz, Poland
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Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland
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Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
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Chair and Department of Medical Microbiology, Medical University of Lublin, 20-093 Lublin, Poland
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Author to whom correspondence should be addressed.
Academic Editors: Waltraud C. Schrottmaier and Marion Mussbacher
Biology 2022, 11(5), 644; https://doi.org/10.3390/biology11050644
Received: 18 March 2022 / Revised: 19 April 2022 / Accepted: 20 April 2022 / Published: 22 April 2022
(This article belongs to the Special Issue Platelets – Fine Tuners of Vascular and Inflammatory Diseases)
Acute Coronary Syndrome is a disease of the circulatory system characterized by the partial or complete blockage of coronary arteries. In thrombosis, a major role is played by platelets—the smallest, anucleate, morphotic elements in the bloodstream. Platelets are involved in the process of hemostasis, which ensures the continuity of the blood vessel by forming a clot that prevents blood loss. Unique structures of blood platelets ensure their high reactivity in the vascular microenvironment due to the interaction with many biologically active molecules, which is recognized by the surface receptors. The research carried out in the manuscript is aimed at detecting potential changes at the molecular level in platelet surface receptors that could constitute the potential importance of the occurrence of Acute Coronary Syndrome. The obtained results indicate that the P2Y12 receptor, which is the main target of antiplatelet therapy, is expressed more frequently among patients. In addition, we have shown that at the genetic level, quantitative changes also occur in the case of other receptors that are important in the activation of platelets. The following manuscript suggests the potential mechanisms responsible for the differences between patients and healthy donors due to a better understanding of the molecular causes of Acute Coronary Syndrome pathogenesis.
The pathological conditions caused by blood platelet activation constitute a fundamental core in the pathogenesis of Acute Coronary Syndrome (ACS). The hyperactivity of platelets in ACS is well-documented, but there is still little research into the molecular basis of phenotypic changes in platelet functionality. To expand the knowledge of this phenomenon, we analyzed the disturbances in the expression of several key platelet receptors and the aspect of regulating potential abnormalities. Platelet surface receptors are responsible for maintaining the hemostatic balance, platelet interaction with immune cells, and support of the coagulation cascade leading to occlusion of the vessel lumen. Due to their prominent role, platelet receptors constitute a major target in pharmacological treatment. Our work aimed to identify the molecular alteration of platelet surface receptors, which showed augmented mRNA expression of P2Y12, GP1BB, ITGA2B, and ITGB3 and increased protein concentrations of P2Y12 and GP IIb/IIIa in ACS. The upregulation of the P2Y12 level was also confirmed by confocal and cytometric visualization. Furthermore, we evaluated the expression of two microRNAs: miR-223-3p and miR-126-3p, which were suggested to regulate platelet P2Y12 expression. Results of our study present new insight into the molecular background of ACS. View Full-Text
Keywords: blood platelets; acute coronary syndrome; surface receptors; miRNA; P2Y12; GP IIb/IIIa blood platelets; acute coronary syndrome; surface receptors; miRNA; P2Y12; GP IIb/IIIa
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MDPI and ACS Style

Szelenberger, R.; Karbownik, M.S.; Kacprzak, M.; Synowiec, E.; Michlewska, S.; Bijak, M.; Zielińska, M.; Olender, A.; Saluk-Bijak, J. Dysregulation in the Expression of Platelet Surface Receptors in Acute Coronary Syndrome Patients—Emphasis on P2Y12. Biology 2022, 11, 644. https://doi.org/10.3390/biology11050644

AMA Style

Szelenberger R, Karbownik MS, Kacprzak M, Synowiec E, Michlewska S, Bijak M, Zielińska M, Olender A, Saluk-Bijak J. Dysregulation in the Expression of Platelet Surface Receptors in Acute Coronary Syndrome Patients—Emphasis on P2Y12. Biology. 2022; 11(5):644. https://doi.org/10.3390/biology11050644

Chicago/Turabian Style

Szelenberger, Rafał, Michał Seweryn Karbownik, Michał Kacprzak, Ewelina Synowiec, Sylwia Michlewska, Michał Bijak, Marzenna Zielińska, Alina Olender, and Joanna Saluk-Bijak. 2022. "Dysregulation in the Expression of Platelet Surface Receptors in Acute Coronary Syndrome Patients—Emphasis on P2Y12" Biology 11, no. 5: 644. https://doi.org/10.3390/biology11050644

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