Stk10 Deficiency in Mice Promotes Tumor Growth by Dysregulating the Tumor Microenvironment
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Tumor Cell Lines and Mice
2.2. Bioinformatic Analysis
2.3. RNA Extraction and qRT-PCR Analyses
2.4. Label of RM-1 Cells with Luciferase
2.5. In Vivo Tumorigenicity Assay
2.6. In Vivo Bioluminescence Assay
2.7. Immunohistochemistry (IHC) and Immunofluorescence (IF) Staining
2.8. Flow Cytometry Analysis
2.9. Western Blot Analysis
2.10. Tube-Formation Assay
2.11. Statistical Analysis
3. Results
3.1. STK10 Was Associated with the Infiltrated Immune Cells in Prostate Cancer
3.2. Generation of Stk10-Deficient Mice
3.3. RM-1 Tumor Growth Was Promoted in Stk10 Knockout Mice
3.4. Stk10−/− Mice Show Tumors with Decreased Infiltration of Activated and Effector CTLs
3.5. Stk10 Knockout Induces Angiogenesis in the Tumor Tissue
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ma, J.-X.; Xu, D.-D.; Lu, S.-Y.; Wang, Q.-L.; Zhang, L.; Guo, R.; Tang, L.-Y.; Shen, Y.; Shen, C.-L.; Wang, J.-J.; et al. Stk10 Deficiency in Mice Promotes Tumor Growth by Dysregulating the Tumor Microenvironment. Biology 2022, 11, 1668. https://doi.org/10.3390/biology11111668
Ma J-X, Xu D-D, Lu S-Y, Wang Q-L, Zhang L, Guo R, Tang L-Y, Shen Y, Shen C-L, Wang J-J, et al. Stk10 Deficiency in Mice Promotes Tumor Growth by Dysregulating the Tumor Microenvironment. Biology. 2022; 11(11):1668. https://doi.org/10.3390/biology11111668
Chicago/Turabian StyleMa, Jin-Xia, Dan-Dan Xu, Shun-Yuan Lu, Qian-Lan Wang, Lu Zhang, Rui Guo, Ling-Yun Tang, Yan Shen, Chun-Ling Shen, Jin-Jin Wang, and et al. 2022. "Stk10 Deficiency in Mice Promotes Tumor Growth by Dysregulating the Tumor Microenvironment" Biology 11, no. 11: 1668. https://doi.org/10.3390/biology11111668