The Northern Root-Knot Nematode Meloidogyne hapla: New Host Records in Portugal
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling and Nematode Isolates
2.2. Morphological Characterization
2.3. Molecular Characterization
3. Results
3.1. Distribution
3.2. Symptoms
Crop | Above-Ground Symptoms (Figure 2) | Below-Ground Symptoms (Figure 3) | PPNs Present in Soil |
---|---|---|---|
Grapevine | Drying of the bunches Yellowing Poor growth | Presence of galls and egg masses | 70 J2 of Meloidogyne/400 mL No other active PPNs were found in the soil extractions |
Eucalyptus | Yellowing Stunting Dead plants | ---- | 70 J2 of Meloidogyne/400 mL No other active PPNs were found in the soil extractions |
Potato | Poor growth | Presence of galls and egg masses | 80 J2 of Meloidogyne/400 mL No other active PPNs were found in the soil extractions |
3.3. Morphological Characterization
3.4. Molecular Characterization
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Code | Year | District | County | Crop |
---|---|---|---|---|
SV1943 | 2019 | Beja | Odemira | Grapevine |
SV55 | 2020 | Madeira Island | Santana | Grapevine |
SV1981 | 2020 | Beja | Odemira | Eucalyptus |
SV1357 | 2021 | Viana de Castelo | Melgaço | Potato |
SV2180 | 2021 | Porto | Vila do Conde | Potato |
SV352 | 2022 | Setúbal | Montijo | Grapevine |
SV12446 | 2022 | Porto | Amarante | Potato |
SV12447 | 2022 | Porto | Amarante | Potato |
SV13382 | 2022 | Porto | Baião | Potato |
Species | Isolate/Year | Country | Host | GenBank Accession |
---|---|---|---|---|
M. hapla | SV1943/2019 | Portugal | Grapevine | OP364011 |
M. hapla | SV55/2020 | Portugal | Grapevine | OP364012 |
M. hapla | SV1981/2020 | Portugal | Eucalyptus | OP364010 |
M. hapla | SV1357/2021 | Portugal | Potato | OP364014 |
M. hapla | SV2180/2021 | Portugal | Potato | OP364015 |
M. hapla | SV352/2022 | Portugal | Grapevine | OP364013 |
M. hapla | SV12446/2022 | Portugal | Potato | OP364017 |
M. hapla | SV12447/2022 | Portugal | Potato | OP364016 |
M. hapla | SV13382/2022 | Portugal | Potato | OP364018 |
M. hapla | 4179/2008 | N. Ireland | Turf grasses | EU908052 |
M. hapla | n.a | Hawaii | Spanish needle | AY528419 |
M. hapla | Kiyokawa 1/2018 | Japan | Tomato | LC030357 |
M. hapla | n.a | China | Carrot | OM854803 |
M. hapla | MH1/2021 | India | Carrot | MZ964890 |
M. hapla | Limpopo/2020 | South Africa | Kiwifruit | MT256109 |
M. hapla | F3-25/50/2022 | USA | Strawberry | OP218878 |
M. hapla | RT83/2019 | USA | Knockout Rose | MK102780 |
M. hapla | V70/2018 | Spain | Wild olive | MH011978 |
M. fallax | 824,Clone8/2014 | USA | Turf grasses | KC241961 |
M. fallax | 825,Clone7/2014 | USA | Turf grasses | KC241960 |
M. fallax | n.a | Netherlands | Beetroot | AY281853 |
M. chitwoodi | 728-1/2020 | USA | Turf grasses | JN157868 |
M. chitwoodi | n.a | Netherlands | Wheat | AY281852 |
M. chitwoodi | PpKL5/2017 | Tunisia | Fababean | FJ712996 |
M. incognita | SS62516/2019 | South Korea | Pepper | MK188478 |
M. incognita | DW7336/2019 | South Korea | Cucucmber | MK188464 |
M. incognita | GXM03/2018 | China | Gynochtodes | MH756120 |
M. arenaria | GS0488/2019 | South Korea | Melon | MK188477 |
M. arenaria | 4564/2018 | Vietnam | Black pepper | MK018123 |
M. arenaria | Tuku/2014 | Taiwan | Peanut | KJ572384 |
M. javanica | XSBN-1/2016 | China | Hemp | KX646187 |
M. javanica | XSYT01/2013 | China | Hemp | KC953091 |
M. javanica | CN0027/2020 | Brazil | Zinnia | MT337435 |
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Rusinque, L.; Nóbrega, F.; Serra, C.; Inácio, M.L. The Northern Root-Knot Nematode Meloidogyne hapla: New Host Records in Portugal. Biology 2022, 11, 1567. https://doi.org/10.3390/biology11111567
Rusinque L, Nóbrega F, Serra C, Inácio ML. The Northern Root-Knot Nematode Meloidogyne hapla: New Host Records in Portugal. Biology. 2022; 11(11):1567. https://doi.org/10.3390/biology11111567
Chicago/Turabian StyleRusinque, Leidy, Filomena Nóbrega, Clara Serra, and Maria L. Inácio. 2022. "The Northern Root-Knot Nematode Meloidogyne hapla: New Host Records in Portugal" Biology 11, no. 11: 1567. https://doi.org/10.3390/biology11111567