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Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2

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Biomedical Research Centre of Aragón (CIBA), Fundación Instituto de Investigación Sanitaria Aragón (IIS Aragón), 50009 Zaragoza, Spain
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Instituto de Carboquímica (ICB), Consejo Superior de Investigaciones Científicas (CSIC), 50018 Zaragoza, Spain
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Institute for Biocomputation and Physics of Complex Systems (BIFI), Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, 50018 Zaragoza, Spain
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Department Microbiology, Preventive Medicine and Public Health, University of Zaragoza, 50009 Zaragoza, Spain
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Aragon I+D Foundation (ARAID), 50018 Zaragoza, Spain
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Laboratorio de Microscopías Avanzada (LMA), Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, 50018 Zaragoza, Spain
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Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, School of Dentistry, University of Copenhagen, DK-2200 Copenhagen, Denmark
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Biobanco de Aragón, Instituto Aragonés de Ciencias de la Salud (IACS), 50009 Zaragoza, Spain
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Unidad de Nanotoxicología e Inmunotoxicología (UNATI), Biomedical Research Centre of Aragón (CIBA), Fundación Instituto de Investigación Sanitaria Aragón (IIS Aragón), 50009 Zaragoza, Spain
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Servicio de Microbiología, Hospital Clinico Universitario Lozano Blesa, 50009 Zaragoza, Spain
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Banco de Sangre y Tejidos de Aragón, 50009 Zaragoza, Spain
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Department Animal Pathology, University of Zaragoza, 50013 Zaragoza, Spain
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Servicio de Prevención de Riesgos Laborales, Hospital Clínico Universitario Lozano Blesa, 50009 Zaragoza, Spain
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Department Pharmacology and Physiology, University of Zaragoza, 50013 Zaragoza, Spain
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Servicio de Enfermedades Infecciosas, Hospital Clinico Universitario Lozano Blesa, 50009 Zaragoza, Spain
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Centro de Investigación Biomédicaen Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 50018 Madrid, Spain
*
Authors to whom correspondence should be addressed.
These authors share first authorship.
Academic Editor: Lubna Pinky
Biology 2021, 10(3), 208; https://doi.org/10.3390/biology10030208
Received: 14 February 2021 / Revised: 1 March 2021 / Accepted: 6 March 2021 / Published: 10 March 2021
(This article belongs to the Special Issue Systems Immunology Approaches in Infectious Diseases)
Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Nevertheless, its efficacy in patients with COVID-19 remains uncertain. Thus, the establishment and validation of standardized methods that predict the viral neutralizing (VN) activity of plasma against SARS-CoV-2 is of utmost importance to appraise its therapeutic value. Using an in-house quantitative ELISA test and two independent cohorts with a total of 345 donors, we found that plasma and serum from most convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, with varying concentrations which correlate with previous disease severity and gender. Anti-RBD IgG plasma concentration significantly correlated with the plasma/serum VN activity against SARS-CoV-2 in vitro.
Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T- and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2. View Full-Text
Keywords: coronavirus; SARS-CoV-2; IgG; antibody; convalescent plasma; ELISA coronavirus; SARS-CoV-2; IgG; antibody; convalescent plasma; ELISA
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MDPI and ACS Style

Santiago, L.; Uranga-Murillo, I.; Arias, M.; González-Ramírez, A.M.; Macías-León, J.; Moreo, E.; Redrado, S.; García-García, A.; Taleb, V.; Lira-Navarrete, E.; Hurtado-Guerrero, R.; Aguilo, N.; del Mar Encabo-Berzosa, M.; Hidalgo, S.; Galvez, E.M.; Ramirez-Labrada, A.; de Miguel, D.; Benito, R.; Miranda, P.; Fernández, A.; Domingo, J.M.; Serrano, L.; Yuste, C.; Villanueva-Saz, S.; Paño-Pardo, J.R.; Pardo, J. Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2. Biology 2021, 10, 208. https://doi.org/10.3390/biology10030208

AMA Style

Santiago L, Uranga-Murillo I, Arias M, González-Ramírez AM, Macías-León J, Moreo E, Redrado S, García-García A, Taleb V, Lira-Navarrete E, Hurtado-Guerrero R, Aguilo N, del Mar Encabo-Berzosa M, Hidalgo S, Galvez EM, Ramirez-Labrada A, de Miguel D, Benito R, Miranda P, Fernández A, Domingo JM, Serrano L, Yuste C, Villanueva-Saz S, Paño-Pardo JR, Pardo J. Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2. Biology. 2021; 10(3):208. https://doi.org/10.3390/biology10030208

Chicago/Turabian Style

Santiago, Llipsy, Iratxe Uranga-Murillo, Maykel Arias, Andrés M. González-Ramírez, Javier Macías-León, Eduardo Moreo, Sergio Redrado, Ana García-García, Víctor Taleb, Erandi Lira-Navarrete, Ramón Hurtado-Guerrero, Nacho Aguilo, Maria del Mar Encabo-Berzosa, Sandra Hidalgo, Eva M. Galvez, Ariel Ramirez-Labrada, Diego de Miguel, Rafael Benito, Patricia Miranda, Antonio Fernández, José M. Domingo, Laura Serrano, Cristina Yuste, Sergio Villanueva-Saz, José R. Paño-Pardo, and Julián Pardo. 2021. "Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2" Biology 10, no. 3: 208. https://doi.org/10.3390/biology10030208

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