Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Method
2.1. Reagents and Chemicals
2.2. Magnetospirillum Magneticum AMB-1 Strain Culture Conditions
2.3. Attachment of PEG–Biotin to MTB
2.4. Characterization of Magnetotactic Bacteria and Magnetosomes
2.5. Assessment of MTB/PEG–Biotin Complex Formation
2.6. Evaluation of Bacterial Viability with PEG–Biotin Attachment
2.6.1. THP-1 Cell Culture
2.6.2. CCK-8-Based Cell Viability Assay
2.6.3. THP-1 Cell Association
2.7. Statistical Analysis
3. Results and Discussion
3.1. Characterization of MTB
3.2. Formation of MTB/PEG–Biotin Complex
3.3. Assessing the Bacterial Viability for the MTB/PEG–Biotin Complex
3.4. Biological Application of the MTB/PEG–Biotin Complex
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chaturvedi, R.; Kang, Y.; Eom, Y.; Torati, S.R.; Kim, C. Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties. Biology 2021, 10, 993. https://doi.org/10.3390/biology10100993
Chaturvedi R, Kang Y, Eom Y, Torati SR, Kim C. Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties. Biology. 2021; 10(10):993. https://doi.org/10.3390/biology10100993
Chicago/Turabian StyleChaturvedi, Richa, Yumin Kang, Yunji Eom, Sri Ramulu Torati, and CheolGi Kim. 2021. "Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties" Biology 10, no. 10: 993. https://doi.org/10.3390/biology10100993