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Article

Preliminary in Silico Studies of the Interactions of Certain Genotoxic Azo Dyes with Different Double-Stranded DNA Conformations

by
Erman Salih İstifli
Department of Biology, Faculty of Science and Literature, Cukurova University, Adana TR-01330, Turkey
Colorants 2022, 1(2), 236-255; https://doi.org/10.3390/colorants1020015
Submission received: 20 March 2022 / Revised: 7 June 2022 / Accepted: 10 June 2022 / Published: 14 June 2022
(This article belongs to the Special Issue Colorants: Ancient and Modern)
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Abstract

:
Organic azo dyes, which are widely used in industrial, health and cosmetic fields, pose genotoxic risks due to their chemical structures; however, the molecular details of the undesirable effects of these dyes on DNA have been poorly or insufficiently clarified. In this computational molecular docking study, the DNA binding modes and binding affinities of 14 azo dyes, previously determined to show DNA clastogenicity, were characterized using 2 different double-stranded DNA (dsDNA) conformations (an intact dsDNA and dsDNA with an intercalation gap). In this study, it was determined that 10 out of the 14 genotoxic azo dyes were strong dsDNA minor groove binders, while the remaining ones formed tight binding complexes with dsDNA through intercalation or threading intercalation modes. The azo, nitro, hydroxyl, ammonium, sulfonate, naphthalene, methoxyphenyl, bromine, nitrophenyl, imidazole, amino-phenylethanol and chloro-nitrophenyl groups were found to play primary role in the most favorable binding conformations of these dyes on dsDNA with an affinity ranging from −6.35 kcal/mol to −9.42 kcal/mol. It was determined that dsDNA sequences containing GT dinucleotides are frequently preferred in binding by these dyes, and that rings and polar groups are important features for tight binding with dsDNA. It was concluded that these dyes may be banned, or non-genotoxic congeners should be manufactured with appropriate molecular optimization for the genetic health of the human population and for future generations.

1. Introduction

Azo dyes are the largest colorant group, constituting 70% of all organic dyes produced in the world [1]. Simple synthesis protocols, large structural diversity, high molar extinction coefficients and medium-to-high fastness properties are the basis of such wide use of azo dyes [1]. The first azo dye, aniline yellow, was produced by Mene in 1861 [2]. On the other hand, dye production by diazotization and azo coupling method, as performed in modern industry today, was first performed in 1875 by Caro and Witt in Badische Aniline and Soda-Fabrik (BASF) [3].
Despite their widespread use around the world, serious biological effects of azo dyes, especially carcinogenic, mutagenic and genotoxic effects, have been consistently reported [4,5,6,7,8]. For instance, some industrial azo dyes are illegal additives that are frequently analyzed in foodstuffs. Although they do not have any nutritional value for the human body, they have toxic, carcinogenic and mutagenic effects that seriously harm consumer health [9].
While some azo dyes (4-aminoazobenzene, o-aminoazotoluene, methyl yellow and its derivatives, Sudan azo dyes and para red) are directly carcinogenic [10], others show carcinogenicity in the form of electrophilic metabolites (benzidine, p-phenylenediamine, etc.) [11]. Amido Black 10B, Basic red 51, Basic Brown 17, Disperse Red I, Disperse Red 13, Disperse Orange 1, Amaranth and Allura red were found to significantly increase the frequency of his+ revertants in Salmonella typhimurium, induced primary DNA damage in the comet test and micronucleus formation, and significantly upregulated genes involved in the inflammatory response, cell cycle control and apoptosis [12,13,14,15,16]. Acid Chrome Blue K, on the other hand, showed its genotoxic effect by binding to the grooves of the DNA in supercoiled plasmid pBR322 and by inducing the transition of the molecule to the nicked DNA form [17]. As can be seen, the structural alterations in the DNA molecule induced by azo dyes can adversely affect human health and jeopardize the molecular integrity of this molecule. If the abnormal structural changes that occur in DNA include genes that control the cell cycle, all these mutations, along with the disabling of apoptosis, can lead to the onset of malignant transformation [18,19,20]. Therefore, elucidation of the genotoxic action mechanisms of azo dyes at the molecular level is also of great importance in terms of public health.
DNA molecules are made up of two long polynucleotide chains consisting of four types of nucleotide subunits. Each of these chains is called a “DNA strand”. The hydrogen bonds between the bases of the nucleotides hold the two chains together. Nucleotides consist of a five-carbon sugar attached to phosphate groups and a nitrogen-containing base. Bases in DNA can be adenine (A), thymine (T), cytosine (C) or guanine (G) [21,22,23].
As is known, small molecules interact with DNA through the mechanisms of intercalation or groove recognition [24,25]. Although the major groove of DNA contains more hydrogen bonding donor and acceptor sites, it is less favorable than the minor groove for binding of small molecules, and the majority of small ligands bind to minor grooves of DNA [25,26]. Among these binding modes, intercalation is the mode for small and rigid aromatic molecules to recognize DNA, while shape-selective agents generally bind to DNA through minor grooves similar to a lock-and-key mechanism [26,27]. In this context, although DNA is a known macromolecular target of azo dyes, studies characterizing the interaction of these dyes with DNA at the molecular level are not sufficient [28]. In silico structure–activity models have been acquiring more importance in elucidating the mechanisms of action of genotoxic small molecules. In this context, the molecular docking method offers unique advantages in harmonizing and correlating the data obtained from genotoxicity and cytotoxicity tests with structural information. Using this method, the visualization and analysis of the genotoxic mechanisms of small molecules at the molecular level can be performed reliably [29,30,31].
Therefore, in this study, double-stranded DNA (dsDNA) binding modes and affinities of certain genotoxic azo dyes, which were determined to interact or bind with dsDNA as a result of a detailed literature analysis, were studied by the molecular docking method. Two different dsDNA conformations were used to determine the dominant dsDNA binding modes of azo dyes: an intact dsDNA and dsDNA with a natural intercalation gap. The mechanistic information obtained as a result of this study provides a molecular-scale perspective on the dsDNA-damage-inducing potential of certain azo dyes.

2. Materials and Methods

2.1. Literature Filtering Criteria

Initially, we held the opinion that it would be useful to give information about the selection criteria of certain azo dyes which were simulated for dsDNA interactions in our study. In the literature filtering step, azo dyes showing experimental evidence of interacting with dsDNA were selected. For this purpose:
  • In the Web of Science database, a total of 84 research articles were found by using a combination of keywords “azo dye genotoxicity”.
  • In the second step, among these 84 articles, further filtering was performed by using a combination of keywords, “genotoxicity DNA”; therefore, the total number of articles was reduced to 28. Thus, articles on the studies of azo dyes in which the genotoxic effect occurs due to intermolecular interactions with DNA were selected.
However, as is widely known, a positive result in genotoxicity/clastogenicity assay performed with cellular systems may not necessarily be an indication of the potential of the substance to cause direct DNA damage. Another point is that the induced lesions in DNA in the comet assay can be efficiently repaired by the cell in a short time [32]. Therefore, a second criterion is whether the DNA damage revealed in genotoxicity tests is due to the direct interaction of the test substance with DNA [32,33,34]. Considering this point, the following further criteria were applied to rationalize the exact azo dye–DNA interaction:
  • In vivo micronucleus (MN) test result should be positive;
  • In vivo chromosome aberration (CA) test result should be positive;
  • Both in vitro comet and in vitro MN/CA test results should be positive;
  • Both in vivo comet and in vivo MN/CA test result should be positive;
  • Capability of forming DNA adducts in DNA-binding assays.
As a result, p-aminoazobenzene, o-aminoazotoluene, p-dimethylaminoazobenzene, phenazopyridine, Acid Chrome Blue K, Basic Red 51, Basic Brown 17, Amaranth, Allura Red, C.I. Disperse Blue 291, Amido Black 10B, C.I. Disperse Red 1, Disperse Orange 1 and Disperse Red 13 dyes were selected as certain DNA-reactive molecules to be simulated in molecular docking studies.

2.2. Receptor/Ligand Retrieval

In this study, azo compounds (Figure 1) (p-aminoazobenzene, o-aminoazotoluene, p-dimethylaminoazobenzene, phenazopyridine, Acid Chrome Blue K, Basic Red 51, Basic Brown 17, Amaranth, Allura Red, CI Disperse Blue 291, Amido Black 10B, CI Disperse Red 1, Disperse Orange 1 and Disperse Red 13), that were simulated based on their interactions with dsDNA, were downloaded in the sdf format from the PubChem database, National Library of Medicine (NIH). On the other hand, two receptor dsDNA molecules (PDB ID: 1MKL; resolution: not applicable; PDB ID: 1HJB; resolution: 3.00 Å) was downloaded from the RCSB Protein Data Bank in the pdb format. 1MKL is a 20-nucleotide dsDNA that possesses the intercalated aflatoxin B1 molecule (therefore, an intercalation gap) on its structure. On the other hand, the double-stranded crystallographic 1HJB consists of a total of 52 nucleotides in which the numerical distribution of A, T, G and C bases (14, 14, 12 and 12, respectively) are balanced; therefore, it does not possess a significant bias in favor of any nucleotide. Therefore, the 1HJB model isolated from Homo sapiens can be a preferable crystallographic structure in molecular docking simulations to better reflect the binding site preferences of azo compounds on the DNA molecule. The rationale for choosing two different dsDNA conformations—with and without an intercalation gap—in our study was to determine which binding mode (intercalative and groove binding) of azo dyes were more dominant.

2.3. Control Dockings

Control groups in molecular docking is important for success [35]. For this purpose, aflatoxin B1 (AFB1), a known DNA intercalator/minor groove binding agent, was used as the positive control in our study. In addition, as a negative control, aspirin (acetylsalicylic acid), a non-steroidal anti-inflammatory (NSAID) drug, which is experimentally proven to not interact strongly with DNA and to be non-genotoxic, was used [36,37,38]. Although the intercalative binding modes of both AFB1 and aspirin [39] have been reported in the literature, AFB1 can also interact with dsDNA through the minor groove binding mode [36,40]. Therefore, as described in the previous section, our docking simulations were performed using two different target conformations—dsDNA with an intercalation gap (1MKL) and an intact dsDNA (1HJB)—to avoid possible false positive or false negative binding poses. Thus, the different binding modes of AFB1 and aspirin arising from conformational changes in dsDNA have been revealed, and it has been shown which of these binding modes was energetically more favorable. Therefore, the positive and negative control groups used in our docking experiments ultimately provided a validation of the employed docking protocol.

2.4. Receptor/Ligand Preparation and Molecular Docking

In this study, 14 azo dyes (Table 1) and the control ligands downloaded from PubChem database in the sdf format were geometrically optimized using the Merck Molecular Force Field 94 (MMFF94) in the Avogadro program, and subsequently saved in pdb format [41]. MMFF94 is an efficient force field in the optimization of organic molecules [42] and provides reasonable accuracy in the geometric optimization of azo dyes and their metabolites [43,44]. On the other hand, the crystallographic dsDNA structures (1MKL and 1HJB) used as the target receptors in our study were prepared by removing the bound ligand and protein complexes in the Discovery Visual Studio v16 program and recorded in pdb format prior to docking.
For molecular modelling, the new AutoDock Vina (version 1.2.0, Center of Computational Structural Biology—Scripps Research, La Jolla, CA, USA) was used to perform rigid receptor–flexible ligand docking simulations in our study. Along with AutoDock4 (AD4), AutoDockGPU, AutoDockFR and AutoDock-CrankPep, AutoDock Vina (Vina) is one of the docking engines in the AutoDock Suite and is among the most widely used and successful docking programs in the world. The reasons for this success are its ease of use, speed (up to 100 times faster than AD4) and open source code, both in the suite and when compared with other docking programs [45].
In the molecular docking studies of azo dyes against two different dsDNA conformations, polar hydrogen atoms in receptor and ligand molecules were retained, while nonpolar hydrogens were merged and then the Gasteiger charges of the ligands were calculated with AutoDockTools, as previously described [46]. On the other hand, the Kollmann charges were added for the target receptors. During the docking experiments, all the rotatable bonds of the azo dyes and control ligands were allowed to rotate freely, and the prepared dsDNA and ligand structures were saved in PDBQT format. Two different grid box sizes were determined in dockings with two different dsDNA conformers: 90 × 90 × 90 Å points (x: 118.9; y: 93.2; z: 14.1) for dsDNA with an intercalation gap; 80 × 80 × 160 Å points (x: 24.8; y: 91.3; z: 4.6) for intact dsDNA, using a grid spacing of 0.375 Å. These grid box sizes included the entire dsDNA structure as the search space and adequately covered all the major and minor grooves of these target receptors.
For all ligands and controls, 2 separate docking runs were performed (40 dockings for each ligand with an exhaustiveness value of 200) against 2 different dsDNA conformations. Following docking, all potential binding modes of the ligands were clustered by AutoDock Vina 1.2.0 and were ranked based on the binding affinity (ΔG°; kcal/mol) of the ligand conformation which showed the lowest binding free energy against the dsDNA targets. The Best-ranked conformation of the ligands obtained by AutoDock Vina 1.2.0 among different poses on the targets was visualized and analyzed using Discovery Studio Visualizer v16.

3. Results and Discussion

3.1. Control Dockings

In this study, aflatoxin B1 (AFB1) and aspirin (acetylsalicylic acid) were used as internal controls for the validation of the docking protocol. Control calculations are important to the success of docking experiments; control molecules are useful in distinguishing real active molecules from real inactive molecules [35]. The positive control molecule AFB1 exhibited intercalation and minor groove binding mode in dsDNA (Table 2), and both modes of binding are consistent with the literature [36,40]. However, the dsDNA intercalation mode of AFB1 was energetically more favorable (ΔG° = −8.64 kcal/mol) (Figure 2a, Table 2). The negative control aspirin binds energetically favorably to both dsDNA conformations (intact dsDNA, dsDNA with intercalation gap), whereas it binds more favorably (ΔG° = −6.10 kcal/mol) to dsDNA with an intercalation gap (Figure 2b, Table 2). The docking conformation of aspirin is in agreement with the reported experimental data. A previous spectroscopic and docking study showed that the dominant binding mode of aspirin to DNA was intercalative [39]. Therefore, positive and negative control ligands (AFB1 and aspirin) that we used in our study provided validation for further docking simulations with azo compounds.

3.2. Molecular Docking against Intact dsDNA and dsDNA with Natural Intercalation Gap

In our study, the docking calculations of 14 azo dyes (p-aminoazobenzene, o-aminoazotoluene, p dimethylaminoazobenzene, phenazopyridine, Acid Chrome Blue K, Basic Red 51, Basic Brown 17, Amaranth, Allura Red, C.I. Disperse Blue 291, Amido Black 10B, C.I. Disperse Red 1, Disperse Orange 1, Disperse Red 13), which were confirmed to be genotoxic as a result of a comprehensive literature review, were performed against two different dsDNA conformations: an intact dsDNA and dsDNA with an intercalation gap. Since the docking simulations using a single receptor conformation can be misleading in terms of the real binding mode of the molecule, such an approach could be used as a strategy to determine which binding mode is energetically more favorable.
P-aminoazobenzene interacted with both dsDNA conformations (intact dsDNA and dsDNA with an intercalation gap) with very close binding affinities (ΔG° = −6.45 kcal/mol; ΔG° = −6.49 kcal/mol, respectively) (Table 2). While hydrogen bonds were more numerous in the interaction of p-aminoazobenzene with the minor groove of intact dsDNA, hydrophobic pi–pi stacking interactions played the most important role in binding with dsDNA carrying an intercalation gap (Table 2). While the interaction with the minor groove was through the phenyl, azo and aniline groups of p-aminoazobenzene, azo and aniline groups, on the other hand, played an important role in the snug fit of this ligand into the dsDNA with an intercalation gap (Figure 3a,b and Figure 4a,b). O-aminoazotoluene was slightly favorably bound to dsDNA containing an intercalation gap compared with the intact dsDNA (ΔG° = −6.92 kcal/mol; ΔG° = −6.73 kcal/mol, respectively) (Table 2). Hydrophobic stacked pi–pi and pi–alkyl interactions played the most important role in the interaction of o-aminoazotoluene with dsDNA carrying an intercalation gap. On the other hand, hydrogen bonds played a dominant role in the interaction with the minor groove of intact dsDNA (Table 2). While 2-methyl aniline and 2-methyl phenyl groups were effective in the threading intercalation of o-aminoazotoluene, only the azo (–N=N–) structure was effective in the interaction of this ligand with the minor groove of intact dsDNA (Figure 3c,d and Figure 4c,d). In the interaction of p-dimethylaminoazobenzene with the dsDNA molecule, the dsDNA affinity of the intercalation (threading) and minor groove binding modes were quite close to each other (ΔG° = −6.50 kcal/mol; ΔG° = −6.66 kcal/mol, respectively) (Table 2). While hydrophobic pi–pi interactions played the most important role in the snug fit of this ligand to the dsDNA containing the intercalation gap, the minor groove recognition mode basically occurred through the formation of H bonds (Table 2). Moreover, it was found that the aniline group was effective in the threading type intercalation of p-dimethylaminoazobenzene on dsDNA, while the azo (–N=N–) structure was effective in convergence and binding to DNA via the minor groove recognition (Figure 3e,f and Figure 4e,f). In the intermolecular interaction of phenazopyridine with the dsDNA molecule, the minor groove binding mode was slightly more favorable than the intercalative binding (ΔG° = −6.94 kcal/mol; ΔG° = −6.69 kcal/mol, respectively) (Table 2). While hydrogen bonds played the dominant role in the interaction of phenazopyridine with the minor groove of the dsDNA molecule, H bonds and hydrophobic pi–pi interactions are prominent in the intercalative binding mode of this ligand (Table 2). The minor groove binding mode of this ligand was mediated by azo and amine groups, while azo, pyridine and amine groups played a dominant role in the intercalative binding of phenazopyridine (Figure 3g,h and Figure 4g,h).
In an in vivo comet assay conducted with eight different mouse organs, p-aminoazobenzene, o-aminoazotoluene, p-dimethylaminoazobenzene and phenazopyridine caused DNA fragmentation in the stomach, colon, lung, urinary bladder, brain and bone marrow, and also caused tumor formation in the liver, lung, urinary bladder and colon [47]. While there is no correlation between the genotoxic potency and the number of azo bonds for these four compounds, the exocyclic amino group of these derivatives (even other azo dyes which possess this group) has been shown to be the critical unit for genotoxicity or carcinogenicity, as it undergoes N-oxidation by cytochrome P450 in the liver to form reactive electrophiles that induce the formation of DNA adducts [48,49,50]. This reported literature data is also in agreement with our docking results, as exocyclic amino groups showed efficacy in the most energetically favorable interactions of these four ligands against the dsDNA (Table 2, Figure 3a–h).
In the intermolecular interaction of Acid Chrome Blue K (ACBK) with the dsDNA, the minor groove binding mode was considerably more favorable compared with the intercalative binding (ΔG° = −9.36 kcal/mol; ΔG° = −8.71 kcal/mol, respectively) (Table 2). H bonds played an important role in the minor groove recognition of ACBK with the dsDNA molecule, while H bonds and hydrophobic pi–pi stacking contacts were more prominent in the intercalative (threading) binding mode of this ligand (Table 2). The minor groove binding mode of this ligand was mediated by sulfonic, naphthalene (hydroxyl groups) and phenol groups (hydroxyl groups); meanwhile, sulfonic and naphthalene rings played a dominant role in its intercalative binding mode (Figure 3i,j and Figure 4i,j). Although there are no adequate studies on the genotoxicity of ACBK in the literature, in a DNA binding assay conducted using plasmid pBR322 DNA, it was found that ACBK was snugly fit into the minor groove of DNA, and the hydroxyl and sulfonic acid moieties have been reported to play a primary role in this binding mode [17]. Additionally, it was shown that an efficient DNA strand cleavage occurred in the presence of a high concentration of ACBK in the irradiated plasmid DNA [17]. These experimental results are in excellent agreement with our docking study, since the dsDNA minor groove binding mode of ACBK had occurred primarily through sulfonic acid and hydroxyl groups; furthermore, the minor groove binding mode of ACBK was energetically quite favorable based on the docking experiment (Table 2, Figure 3i,j).
In the intermolecular interaction of Basic Red 51 with the dsDNA molecule, the intercalative (threading) binding mode was more favorable than minor groove recognition (ΔG° = −6.94 kcal/mol; ΔG° = −6.35 kcal/mol, respectively) (Table 2). While H bonds and hydrophobic pi–pi-stacked contacts played an important role in the intercalative binding mode of Basic Red 51 against the dsDNA molecule, H bonds were more prominent in the minor groove binding mode of this ligand (Table 2). The mode of intercalative binding of this ligand to dsDNA was mediated by the imidazole ring, phenyl, and tertiary amine (on the phenyl), while azo and imidazole rings played a dominant role in the minor groove binding (Figure 3k,l and Figure 4k,l). In the in vitro comet and cytokinesis-blocked micronucleus tests performed on HepG2 cells, Basic Red 51 significantly increased DNA damage (DNA fragmentation and micronucleus, respectively) compared with the control group. Researchers have suggested that the resulting clastogenic damage may be partially due to the azo group in the dye [16]. In our docking study, imidazole ring, phenyl and tertiary amine were the groups shown to be effective in intercalation (Figure 3k,l), which is, energetically, the most favorable binding conformation of Basic Red 51 against the dsDNA.
However, considering that the cellular DNA used in the reported study does not contain intercalation gaps, minor groove binding and thus the effect of azo and imidazole groups may also be responsible for the induced genotoxicity in HepG2 cells [16].
In the interaction of Basic Brown 17 with dsDNA, the minor groove recognition was more efficient than the intercalative (threading) binding mode, and in the minor groove recognition mode, Basic Brown 17 showed a very high binding affinity with dsDNA (ΔG° = −8.79 kcal/mol; ΔG° = −8.50 kcal/mol, respectively) (Table 2). While H bonds and electrostatic interactions played the dominant role in the groove recognition mode, hydrophobic pi–pi-stacked contacts and H bonds were active in Basic Brown 17’s intercalative binding mode (Table 2).
The nitro, azo, hydroxyl and ammonium groups were effective in the minor groove recognition of this ligand, whereas the ammonium, naphthalene and its hydroxyl group, and 2-nitroaniline moieties in the molecular structure of Basic Brown 17 played significant role in the intercalative binding mode with dsDNA (Figure 3m,n and Figure 5a,b). Basic Brown 17 significantly induced the formation of DNA fragments at all concentrations used in the in vitro comet test and chromosomal damage in the micronucleus test in HepG2 cells [16]. Researchers attributed DNA damage induced by Basic Brown 17 partially to the azo group (R–N=N–R′) in its structure. These reported results are in agreement with our docking study, as the azo group, as well as the nitro, hydroxyl and ammonium groups, are active in minor groove recognition, which is the most favorable binding mode of this agent with dsDNA (Table 2). The results obtained from the docking of Basic Brown 17 also show that the docking conformational search can produce the correct molecule orientation compatible with the experimental results.
Amaranth’s interaction with dsDNA through the minor groove was considerably stronger compared with the intercalative (threading) mode and exhibited a very high binding affinity for dsDNA in the minor groove recognition mode (ΔG° = −9.42 kcal/mol; ΔG° = −8.74 kcal/mol, respectively) (Table 2). Basically, many H bonds were effective in Amaranth’s snug fit to the minor groove, while a large number of hydrophobic pi–pi contacts and a small amount of H bonds played a role in the intercalation mode (Table 2). While sulfonate, hydroxyl groups and naphthalene rings in its molecular structure played a significant role in the minor groove binding mode of Amaranth, sulfonate and naphthalene rings were effective in the intercalative binding mode (Figure 3o,p and Figure 5c,d). Amaranth can bind to DNA as shown by UV–vis experiments in which a distinct isosbestic point at 297 nm was observed, suggesting binding of the dye to the DNA [51]. Additionally, Amaranth induced chromosomal damage (specially chromatid breaks) in Chinese hamster fibroblast cell line and increased single-strand DNA breakage in Saccharomyces cerevisiae in the comet assay [15,51]. These results are in agreement with our docking study, because Amaranth formed highly stable complexes with both intact dsDNA and the dsDNA carrying an intercalation gap. However, the type of dsDNA damage that may occur (single- or double-strand DNA breaks) depends highly on the cell cycle phase in which Amaranth interacts with cellular DNA.
In the docking simulation of Allura Red, minor groove recognition was more favorable than the intercalative binding mode, and it exhibited a very favorable binding affinity for dsDNA (ΔG° = −8.80 kcal/mol; ΔG° = −8.19 kcal/mol, respectively) (Table 2). While H bonds were mainly active in the conformational fit of Allura Red to the minor groove, a large number of hydrophobic pi–pi contacts and a small amount of H bonds played role in the intercalation mode (Table 2). The sulfonate, azo and naphthalene ring-bound hydroxyl groups in the molecular structure played the main role in Allura Red’s minor groove recognition, while the planar rings of naphthalene and methoxy phenyl were effective in the intercalative binding mode (Figure 3r,s and Figure 5e,f). It has been reported that Allura Red significantly increased DNA fragmentation (single-strand DNA breaks) between 1250 and 5000 microgram/mL concentrations (at 37 °C) compared with the control in the comet assay of Saccharomyces cerevisiae [15]. The dsDNA binding affinity of Allura Red in the docking simulation correlate with the reported formation of experimental DNA breaks. Allura red forms very stable complexes with dsDNA in both the minor groove and intercalative binding modes, and these complexes are able to induce DNA strand breaks which could be due to changes in DNA conformation or DNA torsional tension.
In the docking simulation of C.I. Disperse Blue 291 (CIDB291), intercalation (threading), which is the primary dsDNA binding mode, was more potent than the major groove binding mode (ΔG° = −7.39 kcal/mol; ΔG° = −6.90 kcal/mol, respectively) (Table 2). In the intercalative binding mode of CIDB291, H bonds were mainly active along with hydrophobic pi–pi and pi–alkyl contacts, while H bonds and a small number of hydrophobic pi–pi and pi–alkyl interactions played a role in the major groove binding mode (Table 2). In the intercalative (threading) binding mode of CIDB291, the phenyl ring and the bromine atom attached to it, as well as the amide and methoxy groups attached to the second phenyl ring played significant role, while the phenyl ring, nitro groups, bromine atom and amide group were effective in the major groove binding mode of this dye (Figure 3t,u and Figure 5g,h). According to our docking results, although CIDB291 has the capacity to bind tightly to dsDNA in its native conformation (most probably via intercalation), acetylated hydroxylamines, which are formed as a result of the metabolic process of CIDB291 by nitro reductase and o-acetyl transferases, have been reported to interact with DNA and pose a mutagenic effect [52]. In addition, it has been reported that genotoxic intermediates of this dye may also occur as a result of the metabolism of -OCH3 and -N(CH2CH3)2) substituents by cytochrome P450 [52]. Therefore, in addition to its native conformation, electrophilic nucleophiles (metabolites) that occur as a result of its metabolism in the host organism play the primary role in the genotoxic effect of CIDB291.
In the docking simulation of Amido Black 10B (AB10B), minor groove recognition, the primary dsDNA binding mode, was highly more potent than the intercalative (threading) mode (ΔG° = −9.23 kcal/mol; ΔG° = −8.30 kcal/mol, respectively) (Table 2). In the minor groove binding mode of AB10B, many H bonds as well as hydrophobic pi–pi T-shaped contacts were active, while hydrophobic pi–pi stacking interactions and small number of H bonds played role in the intercalative binding mode of AB10B (Table 2). In the minor groove binding mode of AB10B, the nitrophenyl ring, the naphtalene moiety with bound amino and hydroxyl groups, as well as the second phenyl ring of the molecule played significant role, while the phenyl ring, azo, nitro and amine groups were effective in the intercalative binding mode of this agent (Figure 3v,w and Figure 5i,j). In a recent experimental study, it was reported that AB10B increased his+ revertants in Salmonella TA98 strain in a dose-dependent manner, while significantly increasing single-/double-strand DNA breaks and micronucleus and nuclear bud formation in human liver HepG2 cell line [12]. These results are in good agreement with our docking study. AB10B can induce single- and double-strand breaks due to its strong affinity to dsDNA (ΔG° = −9.23 kcal/mol in the minor groove binding mode), and these breaks give positive results in the comet and micronucleus test. The simultaneous induction of single and double strand breaks by AB10B indicates that this azo dye can induce DNA strand breaks in both G1 and S phases of the cell cycle. However, according to our docking results, it is also possible for AB10B to induce DNA damage by binding to dsDNA in the intercalation mode (ΔG° = −8.30 kcal/mol).
In the docking simulation of C.I. Disperse Red 1 (CIDR1) against dsDNA, it was determined that minor groove recognition was the primary DNA binding mode and was energetically more favorable than the intercalative (threading) binding mode (ΔG° = −7.67 kcal/mol; ΔG° = −6.94 kcal/mol, respectively) (Table 2). In the minor groove binding mode of CIDR1, H bonds were predominant, while hydrophobic pi–pi stacking interactions and one H bond were effective in the intercalative (threading) binding mode of CIDR1 (Table 2). In the minor groove binding mode of CIDR1, the nitrophenyl moiety and phenyl aminoethanol moiety carrying a hydroxyl group played significant role, while only the phenyl group of the nitrophenyl moiety was effective in the intercalative binding mode of this agent with dsDNA (Figure 3x,y and Figure 5k,l). The DNA-damaging mechanism of CIDR1 is still unknown; however, it is thought that the native molecule of this commercial dye as well as the electrolytic oxidation and reduction products affect the double-helix structure of DNA, causing conformational changes and subsequent genomic damage [53,54]. This hypothesis is also consistent with our docking results, as CIDR1 can form a very tight binding complex (ΔG° = −7.67 kcal/mol) with dsDNA in the minor groove recognition mode, which could lead to DNA breaks in either the G1 or S phase (single- and double-strand breaks, respectively) of the cell cycle. Consistent with this hypothesis, it was found that CIDR1 significantly increased the rate of micronucleated polychromatic erythrocytes (MNPCE) in Swiss mice bone marrow cells and primary DNA damage in the comet assay compared with the control [13].
In the molecular docking simulation of Disperse Orange 1 (DO1), performed with two different dsDNA conformations, minor groove recognition and intercalation (threading) binding modes were found to be energetically close to each other and both were favorable (ΔG° = −8.23 kcal/mol; ΔG° = −8.18 kcal/mol, respectively) (Table 2).
In the minor groove binding mode of DO1, H bonds were predominant, while numerous hydrophobic pi–pi stacking interactions and few H bonds were effective in the intercalative (threading) binding mode of DO1 (Table 2). In the minor groove binding mode of DO1, the nitro group bound to nitrophenyl moiety played significant role, while the planar phenyl ring of the same moiety was effective in the intercalative binding mode of DO1 to dsDNA (Figure 3z,z' and Figure 6a,b). Consistent with our docking study, in a UV–vis spectrophotometric study, it was reported that DO1 and its electrolysis product predominantly bound to the immobilized dsDNA via intercalation mode and damaged the biomolecule [53]. Researchers have reported that local structural changes, such as unwinding of the double helix and lengthening of the DNA strand, are the basis of this damage, which, in turn, lead to retardation or inhibition of transcription and replication [53].
When the docking affinities of the last ligand, Disperse Red 13 (DR13) were compared against two different dsDNA conformations, it was determined that the minor groove recognition mode was energetically slightly more favorable than the intercalative (threading) binding mode (ΔG° = −7.68 kcal/mol; ΔG° = −7.27 kcal/mol, respectively) (Table 2). In the minor groove binding mode of DR13, H bonds were effective, while numerous hydrophobic pi–pi stacking, pi–alkyl and pi–sigma interactions as well as one H bond were effective in the intercalative (threading) binding mode of DR13 (Table 2). The nitro group bound to chloro-nitrophenyl moiety and the hydroxyl of phenyl-aminoethanol moiety played significant role in the minor groove binding mode of DR13, while the two phenyl rings of chloro-nitrophenyl and phenyl-aminoethanol moieties were effective in the intercalative binding mode of DR13 to dsDNA (Figure 3z'',z''' and Figure 6c,d). Although there is no mechanistic study of the DNA binding mechanism of DR13, it has been reported that chlorinated DR13 increases DNA damage in the comet test performed with human liver HepG2 cells in a dose-dependent manner [14]. Although the result obtained from this study is also in agreement with our docking study on the basis of DNA reactivity, additional binding studies are needed regarding the actual DNA interaction mode of DR13. In this study, the entire calculated binding affinities of 14 azo dyes by AutoDock Vina 1.2.0 and the structural differences (RMSD—root mean square deviation) between these binding poses against each DNA conformation can be found in the Supplementary Materials Table S1.

3.3. dsDNA Sequence Selectivity of Top-Ranked Azo Dye Conformations

In this docking study, the determined sequence selectivities (versus two different dsDNA conformations) of the 14 azo dyes in their dominant dsDNA recognition modes are given in Table 3. It has been determined that molecules exhibiting intercalation or threading intercalation mode among 14 azo dyes (p-aminoazobenzene, o-aminoazotoluene, Basic Red 51, CIDB291) preferentially show –CG– or –CGA– sequence selectivity on 20-nucleotide double-stranded dsDNA (PDB ID: 1MKL) having an intercalation gap (Table 3). On the other hand, the remaining 10 ligands with the minor groove binding mode being more favorable, (p-dimethylaminoazobenzene, phenazopyridine, Acid Chrome Blue K, Basic Brown 17, Amaranth, Allura Red, Amido Black 10B, CI Disperse Red 1, Disperse Orange 1, Disperse Red 13) specifically and consistently recognized the –TCTGTGGTTG– sequence on the 52-nucleotide double-stranded DNA molecule (PDB ID: 1HJB) and showed –GT– sequence selectivity (p-dimethylaminoazobenzene, phenazopyridine, Acid Chrome Blue K, Basic Brown 17, Amaranth, Amido Black 10B) (Table 3). DNA regions rich in –GT– dinucleotides have been reported as recombinational hotspots [55]. Furthermore, guanine 2-amino group in DNA could be an important contributor in sequence (G-T)-selective DNA binding [56].
In addition, it has been suggested that –GT– sequences (which are more frequent in the euchromatin), found in all eukaryotes, may play a role in the packaging and condensation of DNA into chromosomes, which are supramolecular structures [57,58]. Therefore, DNA regions rich in –GT– dinucleotides may be multifunctional sequences in that they are located in intra- or inter-genic regions where recombination is triggered and they mediate conformational changes of DNA. Binding of the azo dyes at these genomic regions explains why they are able to induce frequent DNA strand breaks, because these dyes, which could bind to DNA in G1 or S phase, induce single- or double-stranded DNA breaks as a result of the collapse of replication fork [59].
In line with our observations on the minor groove recognition by certain azo dyes, it has been reported that the azo dye “carmoisine” showed specific affinity for the DNA minor groove and bound to the AT-rich region in an experimental study using various biophysical techniques [60]. The sequence of DNA used in that study is not identical to the sequence of intact DNA (1hjb) that we selected in our docking study. Thus, the sequence specificity resulting from binding to the minor groove of DNA may differ in DNAs with different nucleotide sequences.
Finally, we think it would be useful to mention the following regarding the docking calculations we employed in our study: in this study, a “rigid receptor–flexible ligand” docking protocol was applied; therefore, a certain degree of flexibility was allowed on the rotatable bonds of azo dyes during docking, while no conformational plasticity could be simulated in two different dsDNA receptors. Therefore, conformational changes in dsDNA before or upon ligand binding could not be taken into account in this study. For this reason, the docking protocol we used can be considered as a representation of the initial static interactions between the azo dyes and dsDNA receptors. In other words, target flexibility should also be provided (e.g., molecular dynamics) in order to more clearly explain the role of structural features of DNA, such as twist, rise or groove width, in ligand binding, and to determine the time-dependent evolution and stability of resulting DNA–ligand interactions [24,61].

4. Conclusions

Azo dyes have very attractive vivid colors and are the most widely used and structurally diverse group of commercial organic dyes [62]. Approximately more than 2000 azo dyes are used today which are colorants of materials such as synthetic and natural textile fibers, plastic, leather, hair dyes, waxes and certain petroleum products [63]. In this computational docking study investigating the dsDNA binding interactions and affinity of 14 genotoxic azo dyes—which have been previously known to negatively interact with dsDNA—we showed that most of these dyes are preferentially minor groove binders; however, intercalative binding is also an effective dsDNA recognition mode of a small number of these azo dyes. We determined that the molecular moiety of these dyes that play a primary role in the most favorable binding conformations with dsDNA are the azo, nitro, hydroxyl, ammonium, sulfonate, naphthalene, methoxyphenyl, bromine, nitrophenyl, imidazole, amino-phenylethanol and chloro-nitrophenyl groups. These dyes, which exhibit binding energies ranging from −6.35 kcal/mol to −9.42 kcal/mol against the dsDNA molecule, show specific affinity for GT-rich regions (Table 2 and Table 3). The ring structures in the molecular scaffolds of these 14 dyes, which have the ability to form tight binding complexes with dsDNA, as well as some polar groups (such as the hydroxyls and sulfonates) are the critical features for genotoxic dsDNA binding. We are of the opinion that these genotoxic azo dyes, which are frequently used in industrial, health and cosmetic fields, may be banned or non-genotoxic congeners should be immediately produced by further molecular optimization.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/colorants1020015/s1, Table S1: Docking results for azo dyes with AutoDock Vina, showing the binding affinity scores for all poses of ligands and the difference in structure (RMSD) against dsDNA with an intercalation gap and intact dsDNA.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on reasonable request from the corresponding author.

Acknowledgments

We thank Cengiz SARIKURKCU for kindly drawing the two-dimensional structures of genotoxic azo dyes with ChemOffice version 19.1.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Two-dimensional chemical structures of genotoxic azo dyes. Structures were drawn using ChemOffice version 19.1.
Figure 1. Two-dimensional chemical structures of genotoxic azo dyes. Structures were drawn using ChemOffice version 19.1.
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Figure 2. Molecular surface view of the best-ranked conformations of aflatoxin B1 (a,b) and aspirin (c,d) on two different conformations of dsDNA (with and without an intercalation gap) and 3D ligand–dsDNA interaction diagrams. Notice that both the controls, aflatoxin B1 and aspirin, are snugly fitted on the dsDNA with an intercalation gap (a,c).
Figure 2. Molecular surface view of the best-ranked conformations of aflatoxin B1 (a,b) and aspirin (c,d) on two different conformations of dsDNA (with and without an intercalation gap) and 3D ligand–dsDNA interaction diagrams. Notice that both the controls, aflatoxin B1 and aspirin, are snugly fitted on the dsDNA with an intercalation gap (a,c).
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Figure 3. Best-ranked conformations of the genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap) (a–z'''). dsDNA molecule is represented as surface and ligands are depicted in stick mode. P-aminoazobenzene (a,b), o-aminoazotoluene (c,d), p-dimethylaminoazobenzene (e,f), phenazopyridine (g,h), Acid Chrome Blue K (i,j), Basic Red 51 (k,l), Basic Brown 17 (m,n), Amaranth (o,p), Allura red (r,s), C.I. Disperse Blue 291 (t,u), Amido Black 10B (v,w), C.I. Disperse Red 1 (x,y), Disperse Orange 1 (z,z'), Disperse Red 13 (z'',z''').
Figure 3. Best-ranked conformations of the genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap) (a–z'''). dsDNA molecule is represented as surface and ligands are depicted in stick mode. P-aminoazobenzene (a,b), o-aminoazotoluene (c,d), p-dimethylaminoazobenzene (e,f), phenazopyridine (g,h), Acid Chrome Blue K (i,j), Basic Red 51 (k,l), Basic Brown 17 (m,n), Amaranth (o,p), Allura red (r,s), C.I. Disperse Blue 291 (t,u), Amido Black 10B (v,w), C.I. Disperse Red 1 (x,y), Disperse Orange 1 (z,z'), Disperse Red 13 (z'',z''').
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Figure 4. Two-dimensional interaction diagrams of genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap). dsDNA structures and ligands are depicted in stick mode.
Figure 4. Two-dimensional interaction diagrams of genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap). dsDNA structures and ligands are depicted in stick mode.
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Figure 5. Two-dimensional interaction diagrams of genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap). dsDNA structures and ligands are depicted in stick mode.
Figure 5. Two-dimensional interaction diagrams of genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap). dsDNA structures and ligands are depicted in stick mode.
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Figure 6. Two-dimensional interaction diagrams of genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap). dsDNA structures and ligands are depicted in stick mode.
Figure 6. Two-dimensional interaction diagrams of genotoxic azo dyes with dsDNA in two distinct conformations (with and without an intercalation gap). dsDNA structures and ligands are depicted in stick mode.
Colorants 01 00015 g006
Table
Table 1. PubChem CID, molecular weight and molecular formula of the genotoxic azo dyes and their metabolites.
Table 1. PubChem CID, molecular weight and molecular formula of the genotoxic azo dyes and their metabolites.
NoCompoundPubChem
CID		Molecular
Weight (g/mol)		Molecular Formula
Genotoxic azo dyes
1p-Aminoazobenzene6051197.24C12H11N3
2o-Aminoazotoluene7340225.29C14H15N3
3p-Dimethylaminoazobenzene6053225.29C14H15N3 or C6H5N=NC6H4N(CH3)2
4Phenazopyridine4756213.24C11H11N5
5Acid Chrome Blue K135659037586.4C16H9N2Na3O12S3
6Basic Red 51166491279.77C13H18ClN5
7Basic Brown 17135515517401.8C19H20ClN5O3
8Amaranth13506604.5C20H11N2O10S3.3Na or
C20H11N2Na3O10S3
9Allura Red33258496.4C18H14N2Na2O8S2
10C.I. Disperse Blue 29192446509.3C19H21BrN6O6
11Amido Black 10B135442942616.5C22H14N6Na2O9S2
12C.I. Disperse Red 117886314.34C16H18N4O3
13Disperse Orange 117414318.3C18H14N4O2
14Disperse Red 1318516348.78C16H17ClN4O3
Table
Table 2. Docking results of 14 genotoxic azo dyes and control compounds showing the binding free energy (BFE), dual binding mode and intermolecular interactions with dsDNA.
Table 2. Docking results of 14 genotoxic azo dyes and control compounds showing the binding free energy (BFE), dual binding mode and intermolecular interactions with dsDNA.
Dye (Ligand)BFE (kcal/mol)Binding ModeIntermolecular Interactions
AFB1
(positive control)		−8.64Intercalation1 carbon–hydrogen bond (Cyt15), 9 pi–pi stacked (Cyt5, Gua6, Gua16)
−8.12Minor groove3 H bonds (Ade11, Gua18)
Aspirin
(negative control)		−6.10Intercalation1 H bond (Gua6), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−5.77Minor groove4 H bonds (Gua18, Thy19, Gua20)
p-Aminoazobenzene−6.49Intercalation2 H bonds (Gua6), 6 pi–pi stacked (Cyt5, Gua6, Gua16, Cyt15)
−6.45Minor groove3 H bonds (Gua12, Cyt16, Thy17), 1 pi–donor H bond (Gua12)
o-Aminoazotoluene−6.92Threading intercalation1 H bond (Gua16), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16), 2 pi–pi T-shaped (Gua6, Ade7), 5 pi–alkyl (Cyt5, Gua6, Cyt15, Gua16)
−6.73Minor groove2 H bonds (Gua12, Thy17)
p-Dimethylaminoazobenzene−6.50Threading intercalation1 carbon–hydrogen bond (Gua16), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16), 1 pi–pi T-shaped (Gua6)
−6.66Minor groove3 H bonds (Ade11, Gua18, Thy19), 1 carbon–hydrogen bond (Ade11)
Phenazopyridine−6.69Intercalation2 H bonds (Cyt15, Gua16), 1 pi–donor H bond (Gua16), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−6.94Minor groove4 H bonds (Gua18, Thy19, Gua20), 1 carbon–hydrogen bond (Ade11)
Acid Chrome Blue K−8.71Threading intercalation4 H bonds (Cyt5, Gua6, Ade7, Cyt15), 1 carbon–hydrogen bond (Cyt15), 11 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−9.36Minor groove6 H bonds (Ade9, Cyt10, Ade13, Gua18, Gua20), 3 carbon–hydrogen bonds (Gua12, Thy19, Gua20), 1 pi–sulfur (Ade9)
Basic Red 51−6.94Threading intercalation2 carbon–hydrogen bonds (Ade7, Gua16), 1 pi–donor hydrogen bond (Gua6), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−6.35Minor groove1 H bond (Gua12), 1 carbon–hydrogen bond (Cyt16)
Basic Brown 17−8.50Threading intercalation2 H bonds (Ade7, Cyt15), 2 carbon–hydrogen bonds (Gua6), 1 pi–donor hydrogen bond (Gua6), 9 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−8.79Minor groove1 attractive charge (Thy19), 6 H bonds (Ade11, Ade13, Cyt16, Thy17, Gua18), 2 carbon–hydrogen bonds (Gua18)
Amaranth−8.74Threading
intercalation		4 H bonds (Gua6, Cyt15, Gua16), 1 pi–sulfur (Ade7), 11 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16), 1 pi–pi T-shaped (Ade7)
−9.42Minor groove5 H bonds (Ade9, Gua12, Thy17, Gua20, Gua21), 1 carbon–hydrogen bond (Gua12), 1 pi–donor hydrogen bond (Ade11), 1 pi–sulfur (Gua20)
Allura Red−8.19Intercalation1 H bond (Cyt15), 1 carbon–hydrogen bond (Gua16), 1 pi–donor hydrogen bond (Gua6), 10 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16), 2 pi–alkyl (Cyt5, Gua6)
−8.80Minor groove5 H bonds (Ade9, Ade11, Gua12, Gua20, Gua21), 1 carbon–hydrogen bond (Gua12), 1 pi–sulfur (Gua20)
C.I. Disperse Blue 291−7.39Threading intercalation2 H bonds (Gua6, Cyt15), 3 carbon–hydrogen bond (Cyt15, Ade17), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16), 3 pi–alkyl (Cyt15, Gua16)
−6.90Major groove4 H bonds (Ade13, Cyt14), 1 pi–pi T-shaped (Gua14), 1 Pi–alkyl (Gua14)
Amido Black 10B−8.30Threading
intercalation		4 H bonds (Cyt15, Gua16), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−9.23Minor groove7 H bonds (Ade5, Thy22, Thy23, Gua24), 2 pi–pi T-shaped (Gua21, Gua24)
C.I. Disperse Red 1−6.94Threading
intercalation		6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−7.67Minor groove3 H bonds (Ade9, Gua20), 1 pi–donor hydrogen bond (Ade11)
Disperse Orange 1−8.18Threading
intercalation		6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16)
−8.23Minor groove3 H bonds (Ade9, Gua20)
Disperse Red 13−7.27Threading
intercalation		1 pi–donor hydrogen bond (Gua6), 1 pi–sigma (Gua16), 6 pi–pi stacked (Cyt5, Gua6, Cyt15, Gua16), 2 pi–alkyl (Cyt15, Gua16)
−7.68Minor groove4 H bonds (Ade9, Gua12, Gua20)
BFE: Binding free energy (kcal/mol).
Table
Table 3. dsDNA sequence selectivities of the most favorable binding modes of 14 azo dyes against two different dsDNA conformations.
Table 3. dsDNA sequence selectivities of the most favorable binding modes of 14 azo dyes against two different dsDNA conformations.
Dye (Ligand)Most Favorable Binding ModeBFE (kcal/mol)dsDNA Sequence Selectivity
P-aminoazobenzeneIntercalation−6.49 Colorants 01 00015 i001
O-aminoazotolueneThreading intercalation−6.92 Colorants 01 00015 i002
P-dimethylaminoazobenzeneMinor groove−6.66 Colorants 01 00015 i003
PhenazopyridineMinor groove−6.94 Colorants 01 00015 i004
Acid Chrome Blue KMinor groove−9.36 Colorants 01 00015 i005
Basic Red 51Threading intercalation−6.94 Colorants 01 00015 i006
Basic Brown 17Minor groove−8.79 Colorants 01 00015 i007
AmaranthMinor groove−9.42 Colorants 01 00015 i008
Allura RedMinor groove−8.80 Colorants 01 00015 i009
C.I. Disperse Blue 291Threading intercalation−7.39 Colorants 01 00015 i010
Amido Black 10BMinor groove−9.23 Colorants 01 00015 i011
C.I. Disperse Red 1Minor groove−7.67 Colorants 01 00015 i012
Disperse Orange 1Minor groove−8.23 Colorants 01 00015 i013
Disperse Red 13Minor groove−7.68 Colorants 01 00015 i014
BFE—binding free energy (kcal/mol).
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İstifli, E.S. Preliminary in Silico Studies of the Interactions of Certain Genotoxic Azo Dyes with Different Double-Stranded DNA Conformations. Colorants 2022, 1, 236-255. https://doi.org/10.3390/colorants1020015

AMA Style

İstifli ES. Preliminary in Silico Studies of the Interactions of Certain Genotoxic Azo Dyes with Different Double-Stranded DNA Conformations. Colorants. 2022; 1(2):236-255. https://doi.org/10.3390/colorants1020015

Chicago/Turabian Style

İstifli, Erman Salih. 2022. "Preliminary in Silico Studies of the Interactions of Certain Genotoxic Azo Dyes with Different Double-Stranded DNA Conformations" Colorants 1, no. 2: 236-255. https://doi.org/10.3390/colorants1020015

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