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Open AccessFeature PaperArticle

Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

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Institute of Biochemistry, Romanian Academy, 060031 Bucharest, Romania
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National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
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Center for Advanced Laser Technologies (CETAL), National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
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Department of Bioengineering, Faculty of Engineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey
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Center of Nanotechnology & Biomaterials Application & Research, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey
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School of Engineering and Design, Brunel University, London UB8 3PH, UK
*
Authors to whom correspondence should be addressed.
Coatings 2019, 9(12), 781; https://doi.org/10.3390/coatings9120781
Received: 27 October 2019 / Revised: 14 November 2019 / Accepted: 18 November 2019 / Published: 21 November 2019
(This article belongs to the Special Issue Synthetic and Biological-Derived Hydroxyapatite Implant Coatings)
This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications. View Full-Text
Keywords: biological-derived hydroxyapatite coatings; lithium doping; pulsed laser deposition; human mesenchymal stem cells; osteoblasts biological-derived hydroxyapatite coatings; lithium doping; pulsed laser deposition; human mesenchymal stem cells; osteoblasts
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MDPI and ACS Style

Florian, P.E.; Duta, L.; Grumezescu, V.; Popescu-Pelin, G.; Popescu, A.C.; Oktar, F.N.; Evans, R.W.; Roseanu Constantinescu, A. Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts. Coatings 2019, 9, 781.

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