Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts
by
Paula E. Florian 1,*
, Liviu Duta 2,*, Valentina Grumezescu 2, Gianina Popescu-Pelin 2, Andrei C. Popescu 3, Faik N. Oktar 4,5, Robert W. Evans 6 and Anca Roseanu Constantinescu 1
Paula E. Florian 1,*, Liviu Duta 2,*, Valentina Grumezescu 2, Gianina Popescu-Pelin 2, Andrei C. Popescu 3, Faik N. Oktar 4,5, Robert W. Evans 6 and Anca Roseanu Constantinescu 1
1
Institute of Biochemistry, Romanian Academy, 060031 Bucharest, Romania
2
National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
3
Center for Advanced Laser Technologies (CETAL), National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
4
Department of Bioengineering, Faculty of Engineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey
5
Center of Nanotechnology & Biomaterials Application & Research, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey
6
School of Engineering and Design, Brunel University, London UB8 3PH, UK
*
Authors to whom correspondence should be addressed.
Coatings 2019, 9(12), 781; https://doi.org/10.3390/coatings9120781
Received: 27 October 2019 / Revised: 14 November 2019 / Accepted: 18 November 2019 / Published: 21 November 2019
(This article belongs to the Special Issue Synthetic and Biological-Derived Hydroxyapatite Implant Coatings)
This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.
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Keywords:
biological-derived hydroxyapatite coatings; lithium doping; pulsed laser deposition; human mesenchymal stem cells; osteoblasts
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Florian, P.E.; Duta, L.; Grumezescu, V.; Popescu-Pelin, G.; Popescu, A.C.; Oktar, F.N.; Evans, R.W.; Roseanu Constantinescu, A. Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts. Coatings 2019, 9, 781.
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