Next Article in Journal
Aluminum Templates of Different Sizes with Micro-, Nano- and Micro/Nano-Structures for Cell Culture
Next Article in Special Issue
Polymeric Antimicrobial Coatings Based on Quaternary Ammonium Compounds
Previous Article in Journal
Effect of Coating Palm Oil Clinker Aggregate on the Engineering Properties of Normal Grade Concrete
Open AccessArticle

Antimicrobial Films Based on Chitosan and Methylcellulose Containing Natamycin for Active Packaging Applications

Department of Analytical Chemistry, Nutrition, and Food Science, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
Department of Veterinary Medicine and Animal Production, University of Naples, 80137 Naples, Italy
Author to whom correspondence should be addressed.
Academic Editors: Sami Rtimi and Stefanos Giannakis
Coatings 2017, 7(10), 177;
Received: 12 September 2017 / Revised: 6 October 2017 / Accepted: 14 October 2017 / Published: 24 October 2017
(This article belongs to the Special Issue Advances in Antimicrobial Coatings)
Biodegradable polymers are gaining interest as antimicrobial carriers in active packaging. In the present study, two active films based on chitosan (1.5% w/v) and methylcellulose (3% w/v) enriched with natamycin were prepared by casting. The antimicrobial’s release behavior was evaluated by immersion of the films in 95% ethanol (v/v) at different temperatures. The natamycin content in the food simulant was determined by reversed-high performance liquid chromatography with diode-array detection (HPLC-DAD). The apparent diffusion (DP) and partition (KP/S) coefficients were calculated using a mathematical model based on Fick’s Second Law. Results showed that the release of natamycin from chitosan based film (DP = 3.61 × 10−13 cm2/s) was slower, when compared with methylcellulose film (DP = 3.20 × 10−8 cm2/s) at the same temperature (p < 0.05). To evaluate the antimicrobial efficiency of active films, cheese samples were completely covered with the films, stored at 20 °C for 7 days, and then analyzed for moulds and yeasts. Microbiological analyses showed a significant reduction in yeasts and moulds (7.91 log CFU/g) in samples treated with chitosan active films (p < 0.05). The good compatibility of natamycin with chitosan, the low Dp, and antimicrobial properties suggested that the film could be favorably used in antimicrobial packagings. View Full-Text
Keywords: active packaging; chitosan; methylcellulose; natamycin active packaging; chitosan; methylcellulose; natamycin
Show Figures

Figure 1

MDPI and ACS Style

Santonicola, S.; García Ibarra, V.; Sendón, R.; Mercogliano, R.; Rodríguez-Bernaldo de Quirós, A. Antimicrobial Films Based on Chitosan and Methylcellulose Containing Natamycin for Active Packaging Applications. Coatings 2017, 7, 177.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

Back to TopTop