Synergistic Effects of Bacteriocin from Lactobacillus panis C-M2 Combined with Dielectric Barrier Discharged Non-Thermal Plasma (DBD-NTP) on Morganella sp. in Aquatic Foods
Abstract
:1. Introduction
2. Results
2.1. Effects of C-M2 and NTPS on the Growth Inhibition of Morganella sp. wf-1
2.1.1. Treatments with Different Concentrations of C-M2
2.1.2. Treatments with Different times of CSP
2.2. Effects of Lactocin C-M2 and NTPS on the Morphology
2.3. Effects of C-M2 and NTPS on the Cell Membrane Permeability
2.4. Effects of Lactocin C-M2 and NTPS on the Cell Membrane Integrity
2.4.1. Released Cell Nucleic Acid, Protein, and K+
2.4.2. Fatty Acid Composition in Cell Membrane
2.4.3. Cell Fluorescent Staining
2.5. Effects of Lactocin C-M2 and NTPS on the DNA Damage of Morganella sp. wf-1
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Bacterial Strains, Culture Condition, and Preparation
4.3. Different Treatments by Lactocin C-M2 and NTPS
4.4. Viable Bacterial Counting
4.5. Transmission Electron Microscopy (TEM)
4.6. Cell Membrane Permeability
4.7. Release of Cell Nucleic Acid, Protein, and K+
4.8. Fatty Acid Composition in Cell Membranes
4.9. Confocal Laser Scanning Microscopy (CLSM)
4.10. DNA Damage in Cells
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Saturated Fatty Acids | CK | C-M2 | NTPS | C-M2 + NTPS | Unsaturated Fatty Acids | CK | C-M2 | NTPS | C-M2 + NTPS |
---|---|---|---|---|---|---|---|---|---|
C4:0 | 5.21 | 1.64 | 6.56 | 5.09 | C14:1 | 6.23 | 41.72 | 43.40 | 46.93 |
C6:0 | 0.98 | 0.39 | 0.40 | 0.00 | C15:1 | 0.24 | 1.64 | 0.07 | 1.18 |
C8:0 | 0.46 | 0.00 | 0.00 | 0.00 | C16:1 | 0.27 | 8.63 | 0.31 | 26.18 |
C10:0 | 0.75 | 0.39 | 0.00 | 0.00 | C17:1 | 0.21 | 0.61 | 0.79 | 0.43 |
C11:0 | 0.48 | 0.00 | 0.00 | 0.00 | C18:1n9t | 4.61 | 2.94 | 2.64 | 2.95 |
C12:0 | 0.27 | 0.00 | 0.14 | 0.00 | C18:1n9c | 45.61 | 25.98 | 28.75 | 8.93 |
C13:0 | 0.28 | 0.74 | 0.21 | 0.00 | C18:2n6t | 0.04 | 0.88 | 2.09 | 0.00 |
C14:0 | 0.42 | 0.99 | 0.21 | 0.00 | C18:3n6 | 0.02 | 0.00 | 0.02 | 0.00 |
C15:0 | 0.11 | 0.27 | 0.18 | 0.30 | C20:1 | 1.32 | 0.46 | 1.09 | 0.38 |
C16:0 | 9.86 | 3.29 | 0.20 | 1.60 | C18:3n3 /C21:0 | 0.52 | 0.00 | 0.27 | 0.00 |
C17:0 | 1.29 | 0.55 | 0.36 | 0.00 | C20:2 | 0.39 | 0.00 | 0.23 | 0.00 |
C18:0 | 7.03 | 4.25 | 4.73 | 4.15 | C20:3n6 | 0.05 | 0.00 | 0.04 | 0.00 |
C20:0 | 3.52 | 3.68 | 4.39 | 1.88 | C22:1n9 | 9.11 | 0.97 | 2.43 | 0.00 |
C22:0 | 0.12 | 0.00 | 0.04 | 0.00 | C20:3n3 /C20:4n6 | 0.06 | 0.00 | 0.07 | 0.00 |
C23:0 | 0.22 | 0.00 | 0.00 | 0.00 | C22:2 | 0.14 | 0.00 | 0.00 | 0.00 |
C24:0 | 0.11 | 0.00 | 0.00 | 0.00 | C20:5n3 | 0.04 | 0.00 | 0.07 | 0.00 |
C24:1 | 0.03 | 0.00 | 0.32 | 0.00 |
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Shan, C.; Wu, H.; Zhou, J.; Yan, W.; Zhang, J.; Liu, X. Synergistic Effects of Bacteriocin from Lactobacillus panis C-M2 Combined with Dielectric Barrier Discharged Non-Thermal Plasma (DBD-NTP) on Morganella sp. in Aquatic Foods. Antibiotics 2020, 9, 593. https://doi.org/10.3390/antibiotics9090593
Shan C, Wu H, Zhou J, Yan W, Zhang J, Liu X. Synergistic Effects of Bacteriocin from Lactobacillus panis C-M2 Combined with Dielectric Barrier Discharged Non-Thermal Plasma (DBD-NTP) on Morganella sp. in Aquatic Foods. Antibiotics. 2020; 9(9):593. https://doi.org/10.3390/antibiotics9090593
Chicago/Turabian StyleShan, Chengjun, Han Wu, Jianzhong Zhou, Wenjing Yan, Jianhao Zhang, and Xiaoli Liu. 2020. "Synergistic Effects of Bacteriocin from Lactobacillus panis C-M2 Combined with Dielectric Barrier Discharged Non-Thermal Plasma (DBD-NTP) on Morganella sp. in Aquatic Foods" Antibiotics 9, no. 9: 593. https://doi.org/10.3390/antibiotics9090593