Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria
Abstract
:1. Introduction
2. Experimental Methodology
2.1. Electrospinning and Heating Protocol
2.2. Characterization of Electrospun TiO2 NFs
2.3. Evaluation of Antibacterial Activity of Electrospun TiO2 NFs
2.3.1. Bacterial Culture
2.3.2. Investigation of Minimum Inhibitory and Minimum Bactericidal Concentration (MIC/MBC) Values of Electrospun TiO2 NFs
2.4. Effect of TiO2 NFs on Biofilm Formation
2.5. Effect of TiO2 NFs on the Morphology of P. aeruginosa and MRSA: SEM Analysis
3. Results and Discussion
3.1. Effects of the Calcining Atmosphere on TiO2 Colour
3.2. Microstructure Analysis of the Prepared NFs
3.3. Wide-Band Gap Analysis of Calcined Electrospun TiO2 NFs
3.4. Antibacterial and Antibiofilm Activity of TiO2 NFs
3.4.1. MIC and MBC
3.4.2. Effects of Electrospun TiO2 NFs on the Morphology of Bacterial Cells
3.4.3. Inhibition of Biofilm Formation by TiO2 NFs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Calcination Conditions | Eg (eV) |
---|---|
As-electrospun | 3.33 |
100% Air | 3.09 |
50% Air and 50% Argon | 2.94 |
25% Air and75% Argon | 2.91 |
100% Argon | 2.18 |
Electrospun TiO2 Nanofibers Code | Calcination Conditions | Methicillin Resistant S. aureus | P. aeruginosa | ||
---|---|---|---|---|---|
MIC | MBC | MIC | MBC | ||
(a) | 100% Air | 7 | 14 | 7 | 14 |
(b) | 50% Air and 50% Argon | 7 | 14 | 7 | 14 |
(c) | 25% Air and 75% Argon | 6 | 12 | 3 | 6 |
(d) | 100% Argon | >16 | >32 | >16 | >32 |
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Ansari, M.A.; Albetran, H.M.; Alheshibri, M.H.; Timoumi, A.; Algarou, N.A.; Akhtar, S.; Slimani, Y.; Almessiere, M.A.; Alahmari, F.S.; Baykal, A.; et al. Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria. Antibiotics 2020, 9, 572. https://doi.org/10.3390/antibiotics9090572
Ansari MA, Albetran HM, Alheshibri MH, Timoumi A, Algarou NA, Akhtar S, Slimani Y, Almessiere MA, Alahmari FS, Baykal A, et al. Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria. Antibiotics. 2020; 9(9):572. https://doi.org/10.3390/antibiotics9090572
Chicago/Turabian StyleAnsari, Mohammad Azam, Hani Manssor Albetran, Muidh Hamed Alheshibri, Abdelmajid Timoumi, Norah Abdullah Algarou, Sultan Akhtar, Yassine Slimani, Munirah Abdullah Almessiere, Fatimah Saad Alahmari, Abdulhadi Baykal, and et al. 2020. "Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria" Antibiotics 9, no. 9: 572. https://doi.org/10.3390/antibiotics9090572