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Open AccessArticle

Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment

1
Microbiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia 11566, Egypt
2
Microbiology and Immunology Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt
3
School of Health Sciences, Division of Pharmacy & Optometry, University of Manchester, Manchester M13 9NT, UK
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(4), 181; https://doi.org/10.3390/antibiotics9040181
Received: 12 March 2020 / Revised: 8 April 2020 / Accepted: 10 April 2020 / Published: 15 April 2020
(This article belongs to the Special Issue Nanoparticles-Based Antimicrobials)
Silver is a potent antimicrobial agent against a variety of microorganisms and once the element has entered the bacterial cell, it accumulates as silver nanoparticles with large surface area causing cell death. At the same time, the bacterial cell becomes a reservoir for silver. This study aims to test the microcidal effect of silver-killed E. coli O104: H4 and its supernatant against fresh viable cells of the same bacterium and some other species, including E. coli O157: H7, Multidrug Resistant (MDR) Pseudomonas aeruginosa and Methicillin Resistant Staphylococcus aureus (MRSA). Silver-killed bacteria were examined by Transmission Electron Microscopy (TEM). Agar well diffusion assay was used to test the antimicrobial efficacy and durability of both pellet suspension and supernatant of silver-killed E. coli O104:H4 against other bacteria. Both silver-killed bacteria and supernatant showed prolonged antimicrobial activity against the tested strains that extended to 40 days. The presence of adsorbed silver nanoparticles on the bacterial cell and inside the cells was verified by TEM. Silver-killed bacteria serve as an efficient sustained release reservoir for exporting the lethal silver cations. This promotes its use as a powerful disinfectant for polluted water and as an effective antibacterial which can be included in wound and burn dressings to overcome the problem of wound contamination. View Full-Text
Keywords: E. coli O104:H4; sustained release; silver nanoparticles; polluted water and burn dressing E. coli O104:H4; sustained release; silver nanoparticles; polluted water and burn dressing
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Mohamed, D.S.; Abd El-Baky, R.M.; Sandle, T.; Mandour, S.A.; Ahmed, E.F. Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment. Antibiotics 2020, 9, 181.

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