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Antibiotics
Volume 9
Issue 12
10.3390/antibiotics9120866
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Article

Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication

by
Carlos C. F. Pleeging
1,2,3,
Tom Coenye
4,
Dimitris Mossialos
5,
Hilde de Rooster
1,
Daniela Chrysostomou
6,
Frank A. D. T. G. Wagener
2 and
Niels A. J. Cremers
7,*
1
Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Ghent, Belgium
2
Department of Dentistry, Orthodontics and Craniofacial Biology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525EX Nijmegen, The Netherlands
3
Dierenkliniek Parkstad, Bautscherweg 56, 6418EM Heerlen, The Netherlands
4
Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
5
Microbial Biotechnology-Molecular Bacteriology-Virology Laboratory, Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis-Mezurlo, 41500 Larissa, Greece
6
Wound Clinic Health@45, Linksfield Road 45, Dowerglen, Johannesburg 1612, South Africa
7
Triticum Exploitatie BV, Sleperweg 44, 6222NK Maastricht, The Netherlands
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(12), 866; https://doi.org/10.3390/antibiotics9120866
Submission received: 18 November 2020 / Revised: 1 December 2020 / Accepted: 2 December 2020 / Published: 4 December 2020
(This article belongs to the Special Issue Antibiotic Tolerance of Bacterial Biofilms)
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Abstract

Biofilms hinder wound healing. Medical-grade honey (MGH) is a promising therapy because of its broad-spectrum antimicrobial activity and the lack of risk for resistance. This study investigated the inhibitory and eradicative activity against multidrug-resistant Pseudomonas aeruginosa biofilms by different established MGH-based wound care formulations. Six different natural wound care products (Medihoney, Revamil, Mebo, Melladerm, L-Mesitran Ointment, and L-Mesitran Soft) were tested in vitro. Most of them contain MGH only, whereas some were supplemented. L-Mesitran Soft demonstrated the most potent antimicrobial activity (6.08-log inhibition and 3.18-log eradication). Other formulations ranged between 0.89-log and 4.80-log inhibition and 0.65-log and 1.66-log eradication. Therefore, the contribution of different ingredients of L-Mesitran Soft was investigated in more detail. The activity of the same batch of raw MGH (1.38-log inhibition and 2.35-log eradication), vitamins C and E (0.95-log inhibition and 0.94-log eradication), and all ingredients except MGH (1.69-log inhibition and 0.75-log eradication) clearly support a synergistic activity of components within the L-Mesitran Soft formulation. Several presented clinical cases illustrate its clinical antimicrobial efficacy against Pseudomonas aeruginosa biofilms. In conclusion, MGH is a potent treatment for Pseudomonas biofilms. L-Mesitran Soft has the strongest antimicrobial activity, which is likely due to the synergistic activity mediated by its supplements.
Keywords: medical-grade honey; supplements; vitamin C; vitamin E; antimicrobial; biofilm; Pseudomonas aeruginosa; wound medical-grade honey; supplements; vitamin C; vitamin E; antimicrobial; biofilm; Pseudomonas aeruginosa; wound

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MDPI and ACS Style

Pleeging, C.C.F.; Coenye, T.; Mossialos, D.; de Rooster, H.; Chrysostomou, D.; Wagener, F.A.D.T.G.; Cremers, N.A.J. Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication. Antibiotics 2020, 9, 866. https://doi.org/10.3390/antibiotics9120866

AMA Style

Pleeging CCF, Coenye T, Mossialos D, de Rooster H, Chrysostomou D, Wagener FADTG, Cremers NAJ. Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication. Antibiotics. 2020; 9(12):866. https://doi.org/10.3390/antibiotics9120866

Chicago/Turabian Style

Pleeging, Carlos C. F., Tom Coenye, Dimitris Mossialos, Hilde de Rooster, Daniela Chrysostomou, Frank A. D. T. G. Wagener, and Niels A. J. Cremers. 2020. "Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication" Antibiotics 9, no. 12: 866. https://doi.org/10.3390/antibiotics9120866

APA Style

Pleeging, C. C. F., Coenye, T., Mossialos, D., de Rooster, H., Chrysostomou, D., Wagener, F. A. D. T. G., & Cremers, N. A. J. (2020). Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication. Antibiotics, 9(12), 866. https://doi.org/10.3390/antibiotics9120866

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