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Antibiotics 2019, 8(1), 4; https://doi.org/10.3390/antibiotics8010004

Characterization of Two New Multidrug-Resistant Strains of Mycobacterium smegmatis: Tools for Routine In Vitro Screening of Novel Anti-Mycobacterial Agents

1
West African Center for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, P. O. Box LG 54, Accra, Ghana
2
Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK
*
Author to whom correspondence should be addressed.
Received: 13 November 2018 / Revised: 7 December 2018 / Accepted: 7 December 2018 / Published: 2 January 2019
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Abstract

Mycobacterium tuberculosis is a pathogen of global public health concern. This threat is exacerbated by the emergence of multidrug-resistant and extremely-drug-resistant strains of the pathogen. We have obtained two distinct clones of multidrug-resistant Mycobacterium smegmatis after gradual exposure of Mycobacterium smegmatis mc2 155 to increasing concentrations of erythromycin. The resulting resistant strains of Mycobacterium smegmatis exhibited robust viability in the presence of high concentrations of erythromycin and were found to be resistant to a wide range of other antimicrobials. They also displayed a unique growth phenotype in comparison to the parental drug-susceptible Mycobacterium smegmatis mc2 155, and a distinct colony morphology in the presence of cholesterol. We propose that these two multidrug-resistant clones of Mycobacterium smegmatis could be used as model organisms at the inceptive phase of routine in vitro screening of novel antimicrobial agents targeted against multidrug-resistant Mycobacterial tuberculosis. View Full-Text
Keywords: antibiotics; antimicrobial resistance; tuberculosis; mycobacteria antibiotics; antimicrobial resistance; tuberculosis; mycobacteria
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Arthur, P.K.; Amarh, V.; Cramer, P.; Arkaifie, G.B.; Blessie, E.J.S.; Fuseini, M.-S.; Carilo, I.; Yeboah, R.; Asare, L.; Robertson, B.D. Characterization of Two New Multidrug-Resistant Strains of Mycobacterium smegmatis: Tools for Routine In Vitro Screening of Novel Anti-Mycobacterial Agents. Antibiotics 2019, 8, 4.

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