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Antibiotics 2016, 5(2), 19;

Insights into the Stress Response Triggered by Kasugamycin in Escherichia coli

Max F. Perutz Laboratories, Center for Molecular Biology, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Vienna Biocenter (VBC), Dr. Bohr-Gasse 9/4, A-1030 Vienna, Austria
Author to whom correspondence should be addressed.
Academic Editor: Claudio O. Gualerzi
Received: 1 April 2016 / Revised: 19 May 2016 / Accepted: 23 May 2016 / Published: 1 June 2016
(This article belongs to the Special Issue Inhibitors of the Translational Apparatus)
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The bacteriostatic aminoglycoside antibiotic kasugamycin inhibits protein synthesis at an initial step without affecting translation elongation. It binds to the mRNA track of the ribosome and prevents formation of the translation initiation complex on canonical mRNAs. In contrast, translation of leaderless mRNAs continues in the presence of the drug in vivo. Previously, we have shown that kasugamycin treatment in E. coli stimulates the formation of protein-depleted ribosomes that are selective for leaderless mRNAs. Here, we provide evidence that prolonged kasugamycin treatment leads to selective synthesis of specific proteins. Our studies indicate that leaderless and short-leadered mRNAs are generated by different molecular mechanisms including alternative transcription and RNA processing. Moreover, we provide evidence for ribosome heterogeneity in response to kasugamycin treatment by alteration of the modification status of the stalk proteins bL7/L12. View Full-Text
Keywords: kasugamycin; translation initiation; leaderless mRNA; Escherichia coli kasugamycin; translation initiation; leaderless mRNA; Escherichia coli

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Müller, C.; Sokol, L.; Vesper, O.; Sauert, M.; Moll, I. Insights into the Stress Response Triggered by Kasugamycin in Escherichia coli. Antibiotics 2016, 5, 19.

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