Genetic Screen Reveals the Role of Purine Metabolism in Staphylococcus aureus Persistence to Rifampicin
Abstract
:1. Introduction
2. Results
2.1. Identification of New Persister Genes
2.2. Mutants Involved in Regulating Purine Biosynthesis are More Susceptible to a Variety of Stresses Including Antibiotics, Heat and Low pH
2.3. Complementation Studies to Confirm the Role of Purine Pathways in Persistence
Primer Name | Sequence | Source or Reference |
---|---|---|
purBF | 5′-GCAAGATCTATGATTGAACGCTATTCTAG-3′ | This study |
purBR | 5′-ACGGAATTCTTATGCTAATCCAGCGCGTTCG-3′ | This study |
purMF | 5′-GCTAGATCTATGTCTAAAGCATATGAACAATC-3′ | This study |
purMR | 5′-ACGGAATTCTTATACCCCCAACAATTCAAT-3′ | This study |
3. Discussion
4. Experimental Section
4.1. Culture Media, Antibiotics, and Chemicals
4.2. Library Screens to Identify Mutants with Defect in Persistence
4.3. Susceptibility of Mutants to Various Antibiotics and Stresses
4.4. Complementation of S. aureus Mutants
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yee, R.; Cui, P.; Shi, W.; Feng, J.; Zhang, Y. Genetic Screen Reveals the Role of Purine Metabolism in Staphylococcus aureus Persistence to Rifampicin. Antibiotics 2015, 4, 627-642. https://doi.org/10.3390/antibiotics4040627
Yee R, Cui P, Shi W, Feng J, Zhang Y. Genetic Screen Reveals the Role of Purine Metabolism in Staphylococcus aureus Persistence to Rifampicin. Antibiotics. 2015; 4(4):627-642. https://doi.org/10.3390/antibiotics4040627
Chicago/Turabian StyleYee, Rebecca, Peng Cui, Wanliang Shi, Jie Feng, and Ying Zhang. 2015. "Genetic Screen Reveals the Role of Purine Metabolism in Staphylococcus aureus Persistence to Rifampicin" Antibiotics 4, no. 4: 627-642. https://doi.org/10.3390/antibiotics4040627