Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections
Abstract
1. Introduction
2. Results
2.1. False Positives
2.2. False Negatives
2.3. Time to Obtain Results
3. Discussion
3.1. Challenges
3.2. Future Investigations
4. Materials and Methods
4.1. Microbiological Culturing
4.2. Nanopore Sequencing
4.3. Bioinformatic Analysis
4.4. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
MSIS | Musculoskeletal Infection Society |
EBJIS | European Bone and Joint Infection Society |
MDT | Multi-Disciplinary Team |
ESR | Erythrocyte Sedimentation Rate |
CRP | C-Reactive Protein |
PCR | Polymerase Chain Reaction |
NHS | National Health Service |
ONT | Oxford Nanopore Technologies |
BLAST | Basic Local Alignment Search Tool |
PJI | Prosthetic Joint Infections |
References
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For PJI (JS) | Antimicrobial (BA) | Coverage (C) | Host Status (H) | Number Patients |
---|---|---|---|---|
J1 | A1 | C1 | H1 | 3 |
J1 | A1 | C1 | H2 | 3 |
J2 | A1 | C1 | H1 | 2 |
J2 | A1 | C1 | H2 | 3 |
J2 | Ax | C1 | H1 | 1 |
J2 | Ax | C1 | H2 | 1 |
J3 | A2 | C1 | H2 | 1 |
J3 | A3 | C1 | H2 | 1 |
Total | 15 |
EBJIS | Nanopore Sequencing | |
---|---|---|
Infected | Not Infected | |
Infected | 11 | 4 |
Not Infected | 1 | 3 |
EBJIS | Microbiological Cultures | |
---|---|---|
Infected | Not Infected | |
Infected | 10 | 5 |
Not Infected | 1 | 3 |
EBJIS | Sequencing + Cultures | |
---|---|---|
Infected | Not Infected | |
Infected | 14 | 1 |
Not Infected | 0 | 4 |
Sample ID | CRP (mg/L) | ESR (mm/hr) | PMN (10 µg/L) | Culture | Bacterial Species | Clinical Notes |
---|---|---|---|---|---|---|
004 | 23 | 65 | 9.5 | Positive | S. aureus, Strepptococ cus | Above-knee amputation |
017 | 39 | 19 | 6.6 | Negative | n/a | Clinically not infected, asthmatic |
018 | 150 | 104 | 5.2 | Negative | Escherichia coli | Persisting infection following TKR |
020 | 42 | 77 | 6.2 | Negative | Escherichia coli | Persisting infection following TKR |
022 | 5 | 8 | n/a | Negative | n/a | Clinically not infected |
023 | 1 | 5 | 3.0 | Negative | n/a | Clinically not infected |
024 | 2 | 20 | 10 | Negative | n/a | Clinically not infected |
026 | 47 | 101 | 6.5 | Positive | E. faecalis | Synovasure positive |
028 | 68 | 47 | 9.2 | Positive | S. aureus | Sepsis episodes |
029 | 68 | 47 | 9.2 | Positive | S. aureus | Sepsis episodes |
031 | 36 | 49 | 3.8 | Positive | S. epidermidis, S. capitis | Persisting infection following TKR |
036 | 2 | 27 | 6 | Positive | S. anginosus, Anaerobes | Poor host with many comorbidities |
038 | 7 | 5 | 3.3 | Negative | n/a | Second stage of revision |
046 | 177 | 83 | n/a | Positive | S. aureus | Persistent delayed-onset PJI |
063 | 25 | 52 | n/a | Positive | S. aureus | Persistent delayed-onset PJI |
065 | 14 | 29 | 7.4 | Negative | n/a | No clear clinical indication of PJI |
074 | 17 | 73 | 5.5 | Positive | Staphylococcus | First stage of two-stage revision |
096 | 26 | 35 | 4.3 | Positive | S. capitis | No comorbidities |
098 | 27 | 34 | 5.5 | Positive | Pseudomonas, E. faecalis | Amputation for infection, previous cultures all negative |
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Wilkinson, H.; Wright, K.; McCarthy, H.S.; Perry, J.; Hulme, C.; Steele, N.; Burston, B.; Townsend, R.; Cool, P. Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections. Antibiotics 2025, 14, 879. https://doi.org/10.3390/antibiotics14090879
Wilkinson H, Wright K, McCarthy HS, Perry J, Hulme C, Steele N, Burston B, Townsend R, Cool P. Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections. Antibiotics. 2025; 14(9):879. https://doi.org/10.3390/antibiotics14090879
Chicago/Turabian StyleWilkinson, Hollie, Karina Wright, Helen S. McCarthy, Jade Perry, Charlotte Hulme, Niall Steele, Benjamin Burston, Rob Townsend, and Paul Cool. 2025. "Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections" Antibiotics 14, no. 9: 879. https://doi.org/10.3390/antibiotics14090879
APA StyleWilkinson, H., Wright, K., McCarthy, H. S., Perry, J., Hulme, C., Steele, N., Burston, B., Townsend, R., & Cool, P. (2025). Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections. Antibiotics, 14(9), 879. https://doi.org/10.3390/antibiotics14090879