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Article

Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamase Producing Enterobacterales in Healthy Community Dogs in Israel

1
Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel
2
Molecular Biology Department, Ariel University, Ariel 4076414, Israel
3
The Dr. Miriam and Sheldon G. Adelson School of Medicine, Ariel University, Ariel 4076414, Israel
4
Hadassah Braun School of Public Health and Community Medicine, Hebrew University of Jerusalem, Jerusalem 9112102, Israel
5
Clinical Microbiology Lab, Meir Medical Center, Kfar Saba 4428164, Israel
6
National Public Health Laboratory, Public Health Services, Ministry of Health, Tel Aviv 6810416, Israel
7
Ilex Labs, Ilex Medical Ltd., 7 Hatnufa St., Petach Yikva 2951025, Israel
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Nikola Puvača
Antibiotics 2022, 11(8), 1069; https://doi.org/10.3390/antibiotics11081069
Received: 3 July 2022 / Revised: 1 August 2022 / Accepted: 3 August 2022 / Published: 7 August 2022
Background: antimicrobial resistance is a global problem in human and veterinary medicine. We aimed to investigate the extended spectrum β-lactamase-producing Enterobacterales (ESBL-PE) gut colonization in healthy community dogs in Israel. Methods: Rectal swabs were sampled from 145 healthy dogs, enriched, plated on selective plates, sub-cultured to obtain pure cultures, and ESBL production was confirmed. Bacterial species and antibiotic susceptibility profiles were identified. WGS was performed on all of the ESBL-PE isolates and their resistomes were identified in silico. Owners’ questionnaires were collected for risk factor analysis. Results: ESBL-PE gut colonization rate was 6.2% (n = 9/145, 95% CI 2.9–11.5). Overall, ten isolates were detected (one dog had two isolates); the main species was Escherichia coli (eight isolates), belonging to diverse phylogenetic groups—B1, A and C. Two isolates were identified as Citrobacter braakii, and C. portucalensis. A phylogenetic analysis indicated that all of the isolates were genetically unrelated and sporadic. The isolates possessed diverse ESBL genes and antibiotic-resistance gene content, suggesting independent ESBL spread. In a multivariable risk factor analysis, coprophagia was identified as a risk factor for ESBL-PE gut colonization (p = 0.048, aOR = 4.408, 95% CI 1.014–19.169). Conclusions: healthy community dogs may be colonized with ESBL-PE MDR strains, some of which were previously reported in humans, that carry wide and diverse resistomes and may serve as a possible source for AMR. View Full-Text
Keywords: ESBL; Enterobacterales; dogs; coprophagia; antibiotic resistance ESBL; Enterobacterales; dogs; coprophagia; antibiotic resistance
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MDPI and ACS Style

Shnaiderman-Torban, A.; Navon-Venezia, S.; Baron, H.; Abu-Ahmad, W.; Arielly, H.; Zizelski Valenci, G.; Nissan, I.; Paitan, Y.; Steinman, A. Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamase Producing Enterobacterales in Healthy Community Dogs in Israel. Antibiotics 2022, 11, 1069. https://doi.org/10.3390/antibiotics11081069

AMA Style

Shnaiderman-Torban A, Navon-Venezia S, Baron H, Abu-Ahmad W, Arielly H, Zizelski Valenci G, Nissan I, Paitan Y, Steinman A. Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamase Producing Enterobacterales in Healthy Community Dogs in Israel. Antibiotics. 2022; 11(8):1069. https://doi.org/10.3390/antibiotics11081069

Chicago/Turabian Style

Shnaiderman-Torban, Anat, Shiri Navon-Venezia, Hadar Baron, Wiessam Abu-Ahmad, Haya Arielly, Gal Zizelski Valenci, Israel Nissan, Yossi Paitan, and Amir Steinman. 2022. "Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamase Producing Enterobacterales in Healthy Community Dogs in Israel" Antibiotics 11, no. 8: 1069. https://doi.org/10.3390/antibiotics11081069

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