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Antimicrobial and Immunomodulatory Effects of Selected Chemokine and Antimicrobial Peptide on Cytokine Profile during Salmonella Typhimurium Infection in Mouse

1
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, A.P. 510-3, Cuernavaca 62250, Mexico
2
Translational Research Laboratory, National Laboratory of Flow Cytometry, Autonomous University of Chihuahua, Circuito Universitario, Campus II, Chihuahua 31109, Mexico
3
Departamento de Alimentos, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antoquia, AA 1226, Medellín 050010, Colombia
*
Authors to whom correspondence should be addressed.
Academic Editors: Jean-Marc Sabatier, Carlos A. Brizuela and Gabriel Del Río Guerra
Antibiotics 2022, 11(5), 607; https://doi.org/10.3390/antibiotics11050607
Received: 23 February 2022 / Revised: 25 April 2022 / Accepted: 27 April 2022 / Published: 30 April 2022
The antimicrobial and immunomodulatory capacities of the peptide Css54 and the chemokine MCP-1 were tested. The first, a peptide isolated from the venom of the scorpion Centruroides suffusus suffusus was synthesized chemically. In contrast, the second is a monocyte chemoattractant expressed as a recombinant protein in our lab. It was observed in vitro that Css54 inhibited the growth of Salmonella enterica serovar Typhimurium (6.2 µg/mL). At high concentrations, it was toxic to macrophages (25 µg/mL), activated macrophage phagocytosis (1.5 µg/mL), and bound Salmonella LPS (3 µg/mL). On the other hand, the recombinant MCP-1 neither inhibited the growth of Salmonella Typhimurium nor was it toxic to macrophages (up to 25 µg/mL), nor activated macrophage phagocytosis or bound Salmonella LPS (up to 3 µg/mL). Although it was observed in vivo in mice Balb/C that both Css54 and MCP-1 did not resolve the intraperitoneal infection by S. Typhimurium, Css54 decreased the expression of IL-6 and increased IL-10, IL-12p70, and TNF-α levels; meanwhile, MCP-1 decreased the expression of IFN-γ and increased IL-12p70 and TNF-α. It was also observed that the combination of both molecules Css54 and MCP-1 increased the expression of IL-10 and TNF-α. View Full-Text
Keywords: antimicrobial; cytokines; inflammation; peptides; Salmonella enterica serovar Typhimurium antimicrobial; cytokines; inflammation; peptides; Salmonella enterica serovar Typhimurium
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MDPI and ACS Style

Tuxpan-Pérez, A.; Ibarra-Valencia, M.A.; Estrada, B.E.; Clement, H.; Corrales-García, L.L.; Espino-Solis, G.P.; Corzo, G. Antimicrobial and Immunomodulatory Effects of Selected Chemokine and Antimicrobial Peptide on Cytokine Profile during Salmonella Typhimurium Infection in Mouse. Antibiotics 2022, 11, 607. https://doi.org/10.3390/antibiotics11050607

AMA Style

Tuxpan-Pérez A, Ibarra-Valencia MA, Estrada BE, Clement H, Corrales-García LL, Espino-Solis GP, Corzo G. Antimicrobial and Immunomodulatory Effects of Selected Chemokine and Antimicrobial Peptide on Cytokine Profile during Salmonella Typhimurium Infection in Mouse. Antibiotics. 2022; 11(5):607. https://doi.org/10.3390/antibiotics11050607

Chicago/Turabian Style

Tuxpan-Pérez, Astrid, Marco A. Ibarra-Valencia, Blanca E. Estrada, Herlinda Clement, Ligia L. Corrales-García, Gerardo P. Espino-Solis, and Gerardo Corzo. 2022. "Antimicrobial and Immunomodulatory Effects of Selected Chemokine and Antimicrobial Peptide on Cytokine Profile during Salmonella Typhimurium Infection in Mouse" Antibiotics 11, no. 5: 607. https://doi.org/10.3390/antibiotics11050607

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