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Article

Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria

1
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Akad. G. Bonchev Str., 1113 Sofia, Bulgaria
2
University Multiprofile Hospital for Active Treatment Alexandrovska, Medical University, 1 Georgy Sofiiski Str., 1431 Sofia, Bulgaria
*
Author to whom correspondence should be addressed.
Academic Editor: Carmen Losasso
Antibiotics 2021, 10(8), 940; https://doi.org/10.3390/antibiotics10080940
Received: 6 July 2021 / Revised: 23 July 2021 / Accepted: 30 July 2021 / Published: 4 August 2021
(This article belongs to the Special Issue Genomic Analysis and AMR Detection in Pathogenic Bacteria)
Antimicrobial resistance (AMR) is a worldwide health problem affecting humans, animals, and the environment within the framework of the “One Health” concept. The aim of our study was to evaluate the prevalence of pathogenic strains of the species Escherichia coli (E. coli), their AMR profile, and biofilm-forming potential. The isolated strains from three swine faeces and free lagoons (ISO 16654:2001/Amd 1:2017) were confirmed using Phoenix M50 and 16S rDNA PCR. The antibiotic sensitivity to 34 clinically applied antibiotics was determined by Phoenix M50 and the disc diffusion method, according to the protocols of the CLSI and EUCAST. We confirmed the presence of 16 E. coli isolates, of which 87.5% were multi-drug-resistant and 31.25% performed strong biofilms. The possibility for the carrying and transmission of antibiotic-resistance genes to quinolones (qnr), aminoglycosides (aac(3)), β-lactamase-producing plasmid genes ampC, and blaSHV/blaTEM was investigated. We confirmed the carrying of blaSHV/blaTEM in one and ampC in seven isolates. The strains were negative for the virulence genes (ETEC (LT, STa, and F4), EPEC (eae), and STEC/VTEC (stx and stx2all)). The results should contribute to the development of effective measures for limitation and control on the use of antibiotics, which is a key point in the WHO action plan. View Full-Text
Keywords: Escherichia coli; swine faeces and lagoons; antibiotic resistance; PCR methods; Phoenix M50; biofilms Escherichia coli; swine faeces and lagoons; antibiotic resistance; PCR methods; Phoenix M50; biofilms
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MDPI and ACS Style

Dimitrova, L.; Kaleva, M.; Zaharieva, M.M.; Stoykova, C.; Tsvetkova, I.; Angelovska, M.; Ilieva, Y.; Kussovski, V.; Naydenska, S.; Najdenski, H. Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria. Antibiotics 2021, 10, 940. https://doi.org/10.3390/antibiotics10080940

AMA Style

Dimitrova L, Kaleva M, Zaharieva MM, Stoykova C, Tsvetkova I, Angelovska M, Ilieva Y, Kussovski V, Naydenska S, Najdenski H. Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria. Antibiotics. 2021; 10(8):940. https://doi.org/10.3390/antibiotics10080940

Chicago/Turabian Style

Dimitrova, Lyudmila, Mila Kaleva, Maya M. Zaharieva, Christina Stoykova, Iva Tsvetkova, Maya Angelovska, Yana Ilieva, Vesselin Kussovski, Sevda Naydenska, and Hristo Najdenski. 2021. "Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria" Antibiotics 10, no. 8: 940. https://doi.org/10.3390/antibiotics10080940

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