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Article

Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

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Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA
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Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Sao Paulo 18618681, Brazil
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Ross University School of Veterinary Medicine, Basseterre PO Box 334, St. Kitts, West Indies
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Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA
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Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40503, USA
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Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Ulf Magnusson
Antibiotics 2021, 10(5), 490; https://doi.org/10.3390/antibiotics10050490
Received: 24 March 2021 / Revised: 19 April 2021 / Accepted: 21 April 2021 / Published: 23 April 2021
(This article belongs to the Special Issue Antimicrobial Stewardship in Livestock)
Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or -poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre- (−48 and −24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Ilβ, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey’s post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property. View Full-Text
Keywords: endometrium; uterine inflammation; equine; PRP; immunomodulation endometrium; uterine inflammation; equine; PRP; immunomodulation
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MDPI and ACS Style

Segabinazzi, L.G.T.M.; Canisso, I.F.; Podico, G.; Cunha, L.L.; Novello, G.; Rosser, M.F.; Loux, S.C.; Lima, F.S.; Alvarenga, M.A. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics 2021, 10, 490. https://doi.org/10.3390/antibiotics10050490

AMA Style

Segabinazzi LGTM, Canisso IF, Podico G, Cunha LL, Novello G, Rosser MF, Loux SC, Lima FS, Alvarenga MA. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics. 2021; 10(5):490. https://doi.org/10.3390/antibiotics10050490

Chicago/Turabian Style

Segabinazzi, Lorenzo G.T.M., Igor F. Canisso, Giorgia Podico, Lais L. Cunha, Guilherme Novello, Michael F. Rosser, Shavahn C. Loux, Fabio S. Lima, and Marco A. Alvarenga. 2021. "Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares" Antibiotics 10, no. 5: 490. https://doi.org/10.3390/antibiotics10050490

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