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Article

Characterization of blaKPC-2-Carrying Plasmid pR31-KPC from a Pseudomonas aeruginosa Strain Isolated in China

1
State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2
Wuxi Center for Disease Control and Prevention, Wuxi 214023, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Manuela Oliveira, Elisabete Silva and Jonathan Frye
Antibiotics 2021, 10(10), 1234; https://doi.org/10.3390/antibiotics10101234
Received: 11 August 2021 / Revised: 24 September 2021 / Accepted: 9 October 2021 / Published: 11 October 2021
(This article belongs to the Special Issue Antimicrobial Resistance and Virulence - 2nd Volume)
This work aimed to characterize a 29-kb blaKPC-2-carrying plasmid, pR31-KPC, from a multidrug resistant strain of Pseudomonas aeruginosa isolated from the sputum of an elderly patient with multiple chronic conditions in China. The backbone of pR31-KPC is closely related to four other blaKPC-2-carrying plasmids, YLH6_p3, p1011-KPC2, p14057A, and pP23-KPC, none of which have been assigned to any of the known incompatibility groups. Two accessory modules, the IS26-blaKPC-2-IS26 unit and IS26-ΔTn6376-IS26 region, separated by a 5.9-kb backbone region, were identified in pR31-KPC, which was also shown to carry the unique resistance marker blaKPC-2. A comparative study of the above five plasmids showed that p1011-KPC2 may be the most complete plasmid of this group to be reported, while pR31-KPC is the smallest plasmid having lost most of its conjugative region. Regions between the iterons and orf207 in the backbone may be hot spots for the acquisition of exogenous resistance entities. The accessory regions of these plasmids have all undergone several biological events when compared with Tn6296. The further transfer of blaKPC-2 in these plasmids may be initiated by either the Tn3 family or IS26-associated transposition or homologous recombination. The data presented here will contribute to a deeper understanding of blaKPC-2 carrying plasmids in Pseudomonas. View Full-Text
Keywords: Pseudomonas aeruginosa; carbapenem resistance; KPC-2; plasmid Pseudomonas aeruginosa; carbapenem resistance; KPC-2; plasmid
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MDPI and ACS Style

Yuan, M.; Guan, H.; Sha, D.; Cao, W.; Song, X.; Che, J.; Kan, B.; Li, J. Characterization of blaKPC-2-Carrying Plasmid pR31-KPC from a Pseudomonas aeruginosa Strain Isolated in China. Antibiotics 2021, 10, 1234. https://doi.org/10.3390/antibiotics10101234

AMA Style

Yuan M, Guan H, Sha D, Cao W, Song X, Che J, Kan B, Li J. Characterization of blaKPC-2-Carrying Plasmid pR31-KPC from a Pseudomonas aeruginosa Strain Isolated in China. Antibiotics. 2021; 10(10):1234. https://doi.org/10.3390/antibiotics10101234

Chicago/Turabian Style

Yuan, Min, Hongxia Guan, Dan Sha, Wenting Cao, Xiaofeng Song, Jie Che, Biao Kan, and Juan Li. 2021. "Characterization of blaKPC-2-Carrying Plasmid pR31-KPC from a Pseudomonas aeruginosa Strain Isolated in China" Antibiotics 10, no. 10: 1234. https://doi.org/10.3390/antibiotics10101234

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