Activity of Two Antimicrobial Peptides against Enterococcus faecalis in a Model of Biofilm-Mediated Endodontic Infection
Abstract
:1. Introduction
2. Results
2.1. Cytotoxic Effect of The Investigated Peptides
2.2. Activity of KP and L18R against E. faecalis Biofilm on Polystyrene Plates
2.3. Activity of KP and L18R against E. faecalis Biofilm on Hydroxyapatite Disks
3. Discussion
4. Materials and Methods
4.1. Peptides and Bacterial Strain
4.2. Peptide Cytotoxicity Assay
4.3. Treatment of E. faecalis Biofilm Formed on Polystyrene Surfaces
4.4. Treatment of E. faecalis Biofilm Formed on Hydroxyapatite Disks
4.4.1. Confocal Laser Scanning Microscopy
4.4.2. Scanning Electron Microscopy
4.5. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Concentration (µg/mL) | Cell Viability (%) after Treatment with | |
---|---|---|
KP | L18R | |
400 | 104 ± 17 | 127 ± 24 |
200 | 121 ± 15 | 99 ± 20 |
100 | 145 ± 34 | 102 ± 0 |
50 | 120 ± 39 | 120 ± 16 |
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Mergoni, G.; Manfredi, M.; Bertani, P.; Ciociola, T.; Conti, S.; Giovati, L. Activity of Two Antimicrobial Peptides against Enterococcus faecalis in a Model of Biofilm-Mediated Endodontic Infection. Antibiotics 2021, 10, 1220. https://doi.org/10.3390/antibiotics10101220
Mergoni G, Manfredi M, Bertani P, Ciociola T, Conti S, Giovati L. Activity of Two Antimicrobial Peptides against Enterococcus faecalis in a Model of Biofilm-Mediated Endodontic Infection. Antibiotics. 2021; 10(10):1220. https://doi.org/10.3390/antibiotics10101220
Chicago/Turabian StyleMergoni, Giovanni, Maddalena Manfredi, Pio Bertani, Tecla Ciociola, Stefania Conti, and Laura Giovati. 2021. "Activity of Two Antimicrobial Peptides against Enterococcus faecalis in a Model of Biofilm-Mediated Endodontic Infection" Antibiotics 10, no. 10: 1220. https://doi.org/10.3390/antibiotics10101220