RIP3 Associates with RIP1, TRIF, MAVS, and Also IRF3/7 in Host Innate Immune Signaling in Large Yellow Croaker Larimichthys crocea
Abstract
:1. Introduction
2. Results
2.1. Identification and Sequence Analysis of RIP3 in Large Yellow Croakers
2.2. Genomic Organization of RIP3 Genes in Vertebrates
2.3. Subcellular Localization of Lc-RIP3
2.4. Expression Pattern Analysis of Lc-RIP3 in Organs/Tissues
2.5. The Role of Lc-RIP3 in the Regulation of NF-κB Signaling
2.6. The Role of Lc-RIP3 in the Regulation of IRF3 Signaling
3. Discussion
4. Materials and Methods
4.1. Fish, Cell Lines, and Transfection
4.2. Gene Cloning and Construction of Plasmids
4.3. Immune Stimulation and qRT-PCR
4.4. Bioinformatics Analysis
4.5. Confocal Microscopy
4.6. Luciferase Activity Assay
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Common Name | Scientific Name | Accession No. | Length (aa) | Identity | Similarity |
---|---|---|---|---|---|
Japanese flounder | Paralichthys olivaceus | XP_019967771.1 | 494 | 61% | 74% |
Half-smooth tongue sole | Cynoglossus semilaevis | XP_008316289.1 | 496 | 52% | 67% |
Medaka | Oryzias latipes | XP_023821938.1 | 481 | 50% | 66% |
Black carp | Mylopharyngodon piceus | QXJ87326.1 | 426 | 37% | 55% |
Zebrafish | Danio rerio | XP_001343827.1 | 433 | 36% | 52% |
Human | Homo sapiens | NP_006862.2 | 518 | 23% | 40% |
Mouse | Mus musculus | NP_064339.2 | 486 | 24% | 39% |
Primer Name | Sequence (5′–3′) | Application |
---|---|---|
Lc-RIP3-F | ATGGCACTGTCCAGCCAGCT | Lc-RIP3 ORF cloning |
Lc-RIP3-R | GCCAACTCCTCCTGTTTGGTTC | |
pcDNA3.1-RIP3-F | CGGGATCCATGGCACTGTCCAGCCAGCT | pcDNA3.1-RIP3 |
pcDNA3.1-RIP3-R | GGGGTACCGCCAACTCCTCCTGTTTGGTTC | |
pcDNA3.1-TRIF-F | CCGCTCGAGCGATGGCTAGCCGCGAGGGAGAAGA | pcDNA3.1-TRIF |
pcDNA3.1-TRIF-R | CGGGGTACCTTGCTCATCTAAATCATCT | |
pcDNA3.1-MAVS-F | CGCGGATCCATGGCTTCGTTTGCCAGAGACAG | pcDNA3.1-MAVS |
pcDNA3.1-MAVS-R | CCCAAGCTTGTTCTTAAACTTCCACG | |
pcDNA3.1-IRF3-F | CCGGAATTCCAATGGCTTCTCATTCTAAACCTC | pcDNA3.1-IRF3 |
pcDNA3.1-IRF3-R | CCCAAGCTTGTACAGCTCCATCATCT | |
pcDNA3.1-IRF7-F | CGCGGATCCATGGCTCAAAGCCCTCCCAAG | pcDNA3.1-IRF7 |
pcDNA3.1-IRF7-R | CGGGGTACCATAAAGCTCAGCAGCCAG | |
pTurbo-RIP3-F | GGGGTACCGATGGCACTGTCCAGCCAGCT | pTurbo-RIP3-GFP |
pTurbo-RIP3-R | CGGGATCCCGGCCAACTCCTCCTGTTTGGT | |
qRIP3-F | TCAAGGAAGCAGCCCGTATG | qRT-PCR |
qRIP3-R | GAGCCAGTCTGAACACCAACG | |
qβ-actin-F | TTATGAAGGCTATGCCCTGCC | qRT-PCR |
qβ-actin-R | TGAAGGAGTAGCCACGCTCTGT |
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Zou, P.; Li, K.; Li, Y.; Shen, Y.; Zhang, Z.; Wang, Y. RIP3 Associates with RIP1, TRIF, MAVS, and Also IRF3/7 in Host Innate Immune Signaling in Large Yellow Croaker Larimichthys crocea. Antibiotics 2021, 10, 1199. https://doi.org/10.3390/antibiotics10101199
Zou P, Li K, Li Y, Shen Y, Zhang Z, Wang Y. RIP3 Associates with RIP1, TRIF, MAVS, and Also IRF3/7 in Host Innate Immune Signaling in Large Yellow Croaker Larimichthys crocea. Antibiotics. 2021; 10(10):1199. https://doi.org/10.3390/antibiotics10101199
Chicago/Turabian StyleZou, Pengfei, Kaiqing Li, Ying Li, Yingjia Shen, Ziping Zhang, and Yilei Wang. 2021. "RIP3 Associates with RIP1, TRIF, MAVS, and Also IRF3/7 in Host Innate Immune Signaling in Large Yellow Croaker Larimichthys crocea" Antibiotics 10, no. 10: 1199. https://doi.org/10.3390/antibiotics10101199