Next Article in Journal
Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius
Next Article in Special Issue
New Insights on Biofilm Antimicrobial Strategies
Previous Article in Journal
Ceftolozane-Tazobactam Combination Therapy Compared to Ceftolozane-Tazobactam Monotherapy for the Treatment of Severe Infections: A Systematic Review and Meta-Analysis
Previous Article in Special Issue
Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates: In Vivo Virulence Assessment in Galleria mellonella and Potential Therapeutics by Polycationic Oligoethyleneimine
Article

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

1
Human Exposome and Infectious Diseases Network—HEID, School of Nursing of Ribeirão Preto, University of São Paulo, Bandeirantes Avenue 3900, Ribeirão Preto, São Paulo 14040-904, Brazil
2
Department of Dental Materials and Prostheses, School of Dentistry of Ribeirão Preto, University of São Paulo, Café Avenue S/N, Ribeirão Preto, São Paulo 14040-904, Brazil
3
Centre of Biological Engineering—CEB, University of Minho, 4710-057 Braga, Portugal
4
Department of Nursing, University of Brasília, Distrito Federal, Brasília 72220-275, Brazil
5
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Café Avenue S/N, Ribeirão Preto, São Paulo 14040-904, Brazil
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(1), 78; https://doi.org/10.3390/antibiotics10010078
Received: 1 December 2020 / Revised: 4 January 2021 / Accepted: 12 January 2021 / Published: 15 January 2021
(This article belongs to the Special Issue New Insights on Biofilm Antimicrobial Strategies)
Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail’s ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm. View Full-Text
Keywords: bacteriophage; biofilm; Pseudomonas aeruginosa; endotracheal tube bacteriophage; biofilm; Pseudomonas aeruginosa; endotracheal tube
Show Figures

Figure 1

MDPI and ACS Style

Oliveira, V.C.; Macedo, A.P.; Melo, L.D.R.; Santos, S.B.; Hermann, P.R.S.; Silva-Lovato, C.H.; Paranhos, H.F.O.; Andrade, D.; Watanabe, E. Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface. Antibiotics 2021, 10, 78. https://doi.org/10.3390/antibiotics10010078

AMA Style

Oliveira VC, Macedo AP, Melo LDR, Santos SB, Hermann PRS, Silva-Lovato CH, Paranhos HFO, Andrade D, Watanabe E. Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface. Antibiotics. 2021; 10(1):78. https://doi.org/10.3390/antibiotics10010078

Chicago/Turabian Style

Oliveira, Viviane C., Ana P. Macedo, Luís D.R. Melo, Sílvio B. Santos, Paula R.S. Hermann, Cláudia H. Silva-Lovato, Helena F.O. Paranhos, Denise Andrade, and Evandro Watanabe. 2021. "Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface" Antibiotics 10, no. 1: 78. https://doi.org/10.3390/antibiotics10010078

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop