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Biosensors 2018, 8(4), 97; https://doi.org/10.3390/bios8040097

Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique

Optoelectronics Research Centre, University of Southampton, Southampton SO17 1BJ, UK
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Received: 26 September 2018 / Revised: 12 October 2018 / Accepted: 18 October 2018 / Published: 20 October 2018
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Abstract

Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure. View Full-Text
Keywords: lateral-flow device; multiplexed detection; laser direct-write; biosensors; inflammation detection lateral-flow device; multiplexed detection; laser direct-write; biosensors; inflammation detection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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He, P.J.W.; Katis, I.N.; Eason, R.W.; Sones, C.L. Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique. Biosensors 2018, 8, 97.

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