Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
AbstractPaper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure. View Full-Text
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He, P.J.W.; Katis, I.N.; Eason, R.W.; Sones, C.L. Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique. Biosensors 2018, 8, 97.
He PJW, Katis IN, Eason RW, Sones CL. Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique. Biosensors. 2018; 8(4):97.Chicago/Turabian Style
He, Peijun J.W.; Katis, Ioannis N.; Eason, Robert W.; Sones, Collin L. 2018. "Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique." Biosensors 8, no. 4: 97.
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