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A Passive Microfluidic Device Based on Crossflow Filtration for Cell Separation Measurements: A Spectrophotometric Characterization

1
Microelectromechanical Systems Research Unit (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal
2
MEtRICs, Mechanical Engineering Department, University of Minho, 4800-058 Guimarães, Portugal
3
Research Centre in Digitalization and Intelligent Robotics (CeDRI), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
4
CEFT, Faculdade de Engenharia da Universidade do Porto (FEUP) Rua Roberto Frias, 4200-465 Porto, Portugal
*
Authors to whom correspondence should be addressed.
Biosensors 2018, 8(4), 125; https://doi.org/10.3390/bios8040125
Received: 27 October 2018 / Revised: 2 December 2018 / Accepted: 4 December 2018 / Published: 9 December 2018
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Abstract

Microfluidic devices have been widely used as a valuable research tool for diagnostic applications. Particularly, they have been related to the successful detection of different diseases and conditions by assessing the mechanical properties of red blood cells (RBCs). Detecting deformability changes in the cells and being able to separate those cells may be a key factor in assuring the success of detection of some blood diseases with diagnostic devices. To detect and separate the chemically modified RBCs (mimicking disease-infected RBCs) from healthy RBCs, the present work proposes a microfluidic device comprising a sequence of pillars with different gaps and nine different outlets used to evaluate the efficiency of the device by measuring the optical absorption of the collected samples. This latter measurement technique was tested to distinguish between healthy RBCs and RBCs chemically modified with glutaraldehyde. The present study indicates that it was possible to detect a slight differences between the samples using an optical absorption spectrophotometric setup. Hence, the proposed microfluidic device has the potential to perform in one single step a partial passive separation of RBCs based on their deformability. View Full-Text
Keywords: microfluidics; red blood cells; separation; cross-flow filtration; spectrophotometry microfluidics; red blood cells; separation; cross-flow filtration; spectrophotometry
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Faustino, V.; Catarino, S.O.; Pinho, D.; Lima, R.A.; Minas, G. A Passive Microfluidic Device Based on Crossflow Filtration for Cell Separation Measurements: A Spectrophotometric Characterization. Biosensors 2018, 8, 125.

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