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Biosensors 2016, 6(3), 31;

Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds

School of Chemistry and Environment, Faculty of Applied Science, UiTM Kuala Pilah, 72 000 Negeri Sembilan, Malaysia
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Industrial Biotechnology Research Centre, SIRIM Berhad, 1, Persiaran Dato’ Menteri, P.O. Box 7035, Section 2, 40700 Shah Alam, Selangor, Malaysia
Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia
Author to whom correspondence should be addressed.
Academic Editors: Donatella Albanese and Roberto Pilloton
Received: 18 March 2016 / Revised: 6 June 2016 / Accepted: 12 June 2016 / Published: 29 June 2016
(This article belongs to the Special Issue Screen-Printed Electrodes and Sensors)
PDF [5322 KB, uploaded 29 June 2016]


A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075–10 µM and 10–55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days. View Full-Text
Keywords: tyrosinase; zirconium oxide; phenol detection tyrosinase; zirconium oxide; phenol detection

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Ahmad, N.M.; Abdullah, J.; Yusof, N.A.; Ab Rashid, A.H.; Abd Rahman, S.; Hasan, M.R. Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds. Biosensors 2016, 6, 31.

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