Next Article in Journal
Linking Single Domain Antibodies that Recognize Different Epitopes on the Same Target
Previous Article in Journal
Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens
Open AccessArticle

Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

Department of Food Science, University of Massachusetts, 102 Holdsworth Way, Amherst, MA 01003, USA
*
Author to whom correspondence should be addressed.
Biosensors 2012, 2(1), 32-42; https://doi.org/10.3390/bios2010032
Received: 8 December 2011 / Revised: 6 January 2012 / Accepted: 16 January 2012 / Published: 18 January 2012
Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, the causative agent for African sleeping sickness, with relevance in human and animal health in sub-Saharan Africa. The intensity of the chemiluminescent signal was evaluated by using a charge-coupled device as well as a microtiter plate reader. We demonstrated that our lateral flow chemiluminescent assay has a very low limit of detection and is easy to use. The limit of detection was determined to be 0.5 fmols of nucleic acid target. View Full-Text
Keywords: biosensor; lateral flow assay; Trypanosoma; chemiluminescence biosensor; lateral flow assay; Trypanosoma; chemiluminescence
Show Figures

Figure 1

MDPI and ACS Style

Wang, Y.; Fill, C.; Nugen, S.R. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids. Biosensors 2012, 2, 32-42. https://doi.org/10.3390/bios2010032

AMA Style

Wang Y, Fill C, Nugen SR. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids. Biosensors. 2012; 2(1):32-42. https://doi.org/10.3390/bios2010032

Chicago/Turabian Style

Wang, Yuhong; Fill, Catherine; Nugen, Sam R. 2012. "Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids" Biosensors 2, no. 1: 32-42. https://doi.org/10.3390/bios2010032

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop