LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Synthesis of Gold Nanoparticles
2.3. Preparation of Aptamer Solution and NT-proBNP Solution Series
2.4. Procedure for Detecting NT-proBNP Using AuNPs
2.5. Characterization of the Aptamer and NT-proBNP by Circular Dichroism
2.6. Specificity for NT-proBNP
2.7. Collection of Urine and Saliva
2.8. Calibration Curves for NT-proBNP in Biofluids
2.9. Instrumentation
2.10. Statistical Analysis
3. Results and Discussion
3.1. Characterization of Gold Nanoparticles
3.2. Detection of NT-proBNP Using Aptamer and AuNPs
3.3. Interaction of Aptamer with NT-proBNP
3.4. Selectivity Studies
3.5. Effect of Biofluids on NT-proBNP Detection
3.6. Detection of NT-proBNP in Urine
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Method | LOD | Detection Range | Time to Result | Cost (96 Tests) | Ref. |
---|---|---|---|---|---|
ELISA (1) | 11.5 ng·L−1 | 21.9–1400 ng·L−1 | 1 h 30 | 740 euros | [41] |
ELISA (2) | 0.14 µg·L−1 | 0.137–100 µg·L−1 | 4 h 45 | 479 euros | [42] |
ELISA (3) | - | 0.312–10 µg·L−1 | ~9 h | 76 euros | [43] |
Apt-AuNPs | 0.417 µg·L−1 | 0.589–6 µg·L−1 | ~25 min | ~15 euros 1 | This work |
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António, M.; Vitorino, R.; Daniel-da-Silva, A.L. LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP. Biosensors 2023, 13, 736. https://doi.org/10.3390/bios13070736
António M, Vitorino R, Daniel-da-Silva AL. LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP. Biosensors. 2023; 13(7):736. https://doi.org/10.3390/bios13070736
Chicago/Turabian StyleAntónio, Maria, Rui Vitorino, and Ana L. Daniel-da-Silva. 2023. "LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP" Biosensors 13, no. 7: 736. https://doi.org/10.3390/bios13070736