A Label-Free, Mix-and-Detect ssDNA-Binding Assay Based on Cationic Conjugated Polymers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. CCP-Based Binding Assay
2.3. SGI and SYBR Gold-Based Fluorescence Assay
2.4. Quantification of Unlabeled ssDNA on AuNPs
2.5. Quantification of Thiolated ssDNA on AuNPs
3. Results
3.1. The Mechanism of Our CCP-Based ssDNA-Binding Assay
3.2. The Influence of Salt on Our CCP-Based ssDNA-Binding Assay
3.3. Effect of CCP Concentration
3.4. Effect of pH
3.5. Effect of DNA Sequence
3.6. Effect of dsDNA
3.7. Detection of ssDNA on AuNPs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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DNA Type | Method | Binding Efficiency (3 Trials) | Average | ||
---|---|---|---|---|---|
Unlabeled DNA | CCP-based ssDNA-binding assay | 56.0% | 59.3% | 54.4% | 57 ± 3% |
Fluorescence-dye-based method | 59.0% | 59.1% | 59.3% | 59.1 ± 0.2% | |
Thiolated DNA | CCP-based ssDNA-binding assay | 52.2% | 46.4% | 43.6% | 47 ± 4% |
Fluorescence-dye-based method | 46.6% | 47.1% | 47.3% | 47.0 ± 0.4% |
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Zhang, P.; Zandieh, M.; Ding, Y.; Wu, L.; Wang, X.; Liu, J.; Li, Z. A Label-Free, Mix-and-Detect ssDNA-Binding Assay Based on Cationic Conjugated Polymers. Biosensors 2023, 13, 122. https://doi.org/10.3390/bios13010122
Zhang P, Zandieh M, Ding Y, Wu L, Wang X, Liu J, Li Z. A Label-Free, Mix-and-Detect ssDNA-Binding Assay Based on Cationic Conjugated Polymers. Biosensors. 2023; 13(1):122. https://doi.org/10.3390/bios13010122
Chicago/Turabian StyleZhang, Pengbo, Mohamad Zandieh, Yuzhe Ding, Lyuyuan Wu, Xiaoyu Wang, Juewen Liu, and Zhengping Li. 2023. "A Label-Free, Mix-and-Detect ssDNA-Binding Assay Based on Cationic Conjugated Polymers" Biosensors 13, no. 1: 122. https://doi.org/10.3390/bios13010122