Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode
Abstract
:1. Introduction
2. Materials and Methods
2.1. Oligonucleotides and Some Materials
2.2. Synthesis of the Model of SARS-CoV-2 Virions
2.3. Characterization of Nanoparticles Coated with RBD Protein
2.4. SERS-Active Aptasensor Platform
2.5. SERS-Based Substrates
2.6. Characterization of SERS-Based Substrates
3. Results and Discussion
3.1. Fabrication and Characterization of Model of SARS-CoV-2 Virions
3.2. Characterization of the Hybrid Plasmonic Sandwiched Structures
3.3. SERS-Active Aptasensor Platform for the Detection of Model of SARS-CoV-2 Virions Using Hybrid Plasmonic Substrates
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primary Aptamer | Nanoparticles | Secondary Aptamer | Comment | |
---|---|---|---|---|
Sample 1 | Found-SH | RBD NP | RBD-1C-Cy3 | Experiment |
Sample 2 | CompFound-SH | RBD NP | RBD-1C-Cy3 | Control with nonspecific primary oligonucleotide |
Sample 3 | Found-SH | BSA NP | RBD-1C-Cy3 | Control with nonspecific protein instead of RBD |
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Samodelova, M.V.; Kapitanova, O.O.; Meshcheryakova, N.F.; Novikov, S.M.; Yarenkov, N.R.; Streletskii, O.A.; Yakubovsky, D.I.; Grabovenko, F.I.; Zhdanov, G.A.; Arsenin, A.V.; et al. Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode. Biosensors 2022, 12, 768. https://doi.org/10.3390/bios12090768
Samodelova MV, Kapitanova OO, Meshcheryakova NF, Novikov SM, Yarenkov NR, Streletskii OA, Yakubovsky DI, Grabovenko FI, Zhdanov GA, Arsenin AV, et al. Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode. Biosensors. 2022; 12(9):768. https://doi.org/10.3390/bios12090768
Chicago/Turabian StyleSamodelova, Mariia V., Olesya O. Kapitanova, Nadezda F. Meshcheryakova, Sergey. M. Novikov, Nikita R. Yarenkov, Oleg A. Streletskii, Dmitry I. Yakubovsky, Fedor I. Grabovenko, Gleb A. Zhdanov, Aleksey V. Arsenin, and et al. 2022. "Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode" Biosensors 12, no. 9: 768. https://doi.org/10.3390/bios12090768