Single-Particle Optical Imaging for Ultrasensitive Bioanalysis
Abstract
:1. Introduction
2. Ultrasensitive Bioanalysis by Different OSPI Techniques
2.1. Single-Particle Fluorescence Imaging
2.2. Single-Particle ECL Imaging
2.3. Single-Particle SERS Imaging
2.4. Single-Particle Dark-Field Scattering
3. Summery and Outlook
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Methods | Probes | Targets | Linear Range | Detection Limit | Ref. |
---|---|---|---|---|---|
Fluorescence | Cy3, Cy5 | miRNA-21, miRNA-122 | 10 fM–1 nM, 10 fM–1 nM | 5 fM, 5 fM | [83] |
Fluorescence | QDs, Cy5 | miRNA-21 | 1 fM–1 pM | 1 fM | [95] |
Fluorescence | QDs | DNA MTase | 0.001–1 U/mL | 0.0005 U/mL | [99] |
Fluorescence | QDs | miRNA-375 | / | 10 aM | [100] |
Fluorescence | UCNPs | PSA 1 | 0–500 pM | 1 pM | [103] |
Fluorescence | UCNPs | Aflatoxin B1 | 3.31–125 ng/mL | 0.17 ng/mL | [104] |
Fluorescence | UCNPs | PSA | 0.1–1000 pg/mL | 23 fg/mL | [105] |
ECL | RuDSNS/ AuNPs | CK19 2 | 0.01–10 ng/mL | 0.12 pg/mL | [117] |
SERS | Labeled AuNPs | miRNA-21 | 0.1–100 pM | 0.1 pM | [131] |
DFM | AuNPs | SrtA 3 | 0.05–50 nM | 7.9 pM | [141] |
DFM | AuNPs | IL-6 4 | / | 7 pg/mL | [143] |
DFM | AuNPs | miRNA-21 | 10–200 pM | 2 pM | [144] |
DFM | AuNRs, AuNPs | miRNA-21 | 0.02–1 nM | 2 pM | [146] |
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Liu, Y.; Li, B.; Liu, B.; Zhang, K. Single-Particle Optical Imaging for Ultrasensitive Bioanalysis. Biosensors 2022, 12, 1105. https://doi.org/10.3390/bios12121105
Liu Y, Li B, Liu B, Zhang K. Single-Particle Optical Imaging for Ultrasensitive Bioanalysis. Biosensors. 2022; 12(12):1105. https://doi.org/10.3390/bios12121105
Chicago/Turabian StyleLiu, Yujie, Binxiao Li, Baohong Liu, and Kun Zhang. 2022. "Single-Particle Optical Imaging for Ultrasensitive Bioanalysis" Biosensors 12, no. 12: 1105. https://doi.org/10.3390/bios12121105