The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Modification of Haematocrit
2.2.1. Protocol for Reducing Haematocrit
- Measure initial haematocrit (HCT);
- Calculate additional volume of plasma to reduce HCT to desired level using Equation (1):
- 3.
- Separate plasma from whole blood stock (Eppendorf centrifuge: 3000 rpm, 10 min).
- 4.
- Add plasma to whole blood and gently mix with rotation mixer for 30 min.
2.2.2. Protocol for increasing haematocrit
- Measure initial HCT;
- Calculate the reduction in volume of plasma required for the desired HCT level using Equation (2);
- Gently centrifuge whole blood (1000 rpm, 15 min) to separate plasma and red blood cells;
- Pipette off the difference in plasma;
- Gently mix with rotation mixer for 30 min.
2.3. ATR-FTIR Measurements
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rowe, D.J.; Owens, D.R.; Parker, S.L.; Faust, S.N.; Wilkinson, J.S.; Mashanovich, G.Z. The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood. Biosensors 2021, 11, 417. https://doi.org/10.3390/bios11110417
Rowe DJ, Owens DR, Parker SL, Faust SN, Wilkinson JS, Mashanovich GZ. The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood. Biosensors. 2021; 11(11):417. https://doi.org/10.3390/bios11110417
Chicago/Turabian StyleRowe, David J., Daniel R. Owens, Suzanne L. Parker, Saul N. Faust, James S. Wilkinson, and Goran Z. Mashanovich. 2021. "The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood" Biosensors 11, no. 11: 417. https://doi.org/10.3390/bios11110417