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Review

Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments

1
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Cranfield Water Science Institute, Cranfield University, Cranfield MK43 0AL, UK
*
Authors to whom correspondence should be addressed.
Biosensors 2021, 11(10), 352; https://doi.org/10.3390/bios11100352
Received: 12 August 2021 / Revised: 16 September 2021 / Accepted: 21 September 2021 / Published: 23 September 2021
(This article belongs to the Special Issue Biosensors for Diagnosis and Monitoring)
Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted increasing attention; for example, rolling circle amplification (RCA) is an isothermal enzymatic process in which a short nucleic acid primer is amplified to form a long single-stranded nucleic acid using a circular template and special nucleic acid polymerases. Furthermore, this approach can be further engineered into a device for point-of-need monitoring of environmental pollutants. In this paper, we describe the fundamental principles of RCA and the advantages and disadvantages of RCA assays. Then, we discuss the recently developed RCA-based tools for environmental analysis to determine various targets, including heavy metals, organic small molecules, nucleic acids, peptides, proteins, and even microorganisms in aqueous environments. Finally, we summarize the challenges and outline strategies for the advancement of this technique for application in contaminant monitoring. View Full-Text
Keywords: rolling circle amplification; environmental monitoring; heavy metals; organic molecules; microorganisms rolling circle amplification; environmental monitoring; heavy metals; organic molecules; microorganisms
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MDPI and ACS Style

Zhang, K.; Zhang, H.; Cao, H.; Jiang, Y.; Mao, K.; Yang, Z. Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments. Biosensors 2021, 11, 352. https://doi.org/10.3390/bios11100352

AMA Style

Zhang K, Zhang H, Cao H, Jiang Y, Mao K, Yang Z. Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments. Biosensors. 2021; 11(10):352. https://doi.org/10.3390/bios11100352

Chicago/Turabian Style

Zhang, Kuankuan, Hua Zhang, Haorui Cao, Yu Jiang, Kang Mao, and Zhugen Yang. 2021. "Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments" Biosensors 11, no. 10: 352. https://doi.org/10.3390/bios11100352

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