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Open AccessArticle

Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring

1
Swiss Center for Electronics and Microtechnology (CSEM, Landquart), Bahnhofstrasse 1, 7302 Landquart, Switzerland
2
Organs-on-Chip Technologies, ARTORG Center for Biomedical Engineering Research, University of Bern, Murtenstrasse 50, 3008 Bern, Switzerland
*
Author to whom correspondence should be addressed.
Biosensors 2020, 10(9), 125; https://doi.org/10.3390/bios10090125
Received: 14 August 2020 / Revised: 10 September 2020 / Accepted: 11 September 2020 / Published: 13 September 2020
(This article belongs to the Special Issue Novel Materials for Electrochemical Biosensors)
Glucose sensors are potentially useful tools for monitoring the glucose concentration in cell culture medium. Here, we present a new, low-cost, and reproducible sensor based on a cellulose-based material, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidized-cellulose nanocrystals (CNCs). This novel biocompatible and inert nanomaterial is employed as a polymeric matrix to immobilize and stabilize glucose oxidase in the fabrication of a reproducible, operationally stable, highly selective, cost-effective, screen-printed glucose sensor. The sensors have a linear range of 0.1–2 mM (R2 = 0.999) and a sensitivity of 5.7 ± 0.3 µA cm−2∙mM−1. The limit of detection is 0.004 mM, and the limit of quantification is 0.015 mM. The sensor maintains 92.3 % of the initial current response after 30 consecutive measurements in a 1 mM standard glucose solution, and has a shelf life of 1 month while maintaining high selectivity. We demonstrate the practical application of the sensor by monitoring the glucose consumption of a fibroblast cell culture over the course of several days. View Full-Text
Keywords: cellulose nanocrystals; amperometric glucose sensor; cell culture monitoring; screen-printed electrodes; long-term stability cellulose nanocrystals; amperometric glucose sensor; cell culture monitoring; screen-printed electrodes; long-term stability
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MDPI and ACS Style

Tang, Y.; Petropoulos, K.; Kurth, F.; Gao, H.; Migliorelli, D.; Guenat, O.; Generelli, S. Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring. Biosensors 2020, 10, 125. https://doi.org/10.3390/bios10090125

AMA Style

Tang Y, Petropoulos K, Kurth F, Gao H, Migliorelli D, Guenat O, Generelli S. Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring. Biosensors. 2020; 10(9):125. https://doi.org/10.3390/bios10090125

Chicago/Turabian Style

Tang, Ye; Petropoulos, Konstantinos; Kurth, Felix; Gao, Hui; Migliorelli, Davide; Guenat, Olivier; Generelli, Silvia. 2020. "Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring" Biosensors 10, no. 9: 125. https://doi.org/10.3390/bios10090125

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