Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells
Abstract
:1. Introduction
2. Methodology
2.1. Chemicals and Instruments
2.2. ALP Release and Cell Culture
2.3. Stabilization of Graphite Screen-Printed Electrodes
2.4. Optimization of Electrochemical Measurement
2.5. Linearity Performance of ALP Release vs. Cell Number
2.6. Concentration of the Substrate pAPP from Adhesion Cells
2.7. Comparative Study of ALP Activity
3. Results and Discussion
3.1. Stabilization of Graphite Screen-Printed Electrodes
3.2. Optimization of Electrochemical Measurement
3.3. Linearity Performance of ALP Release vs. Cell Number
3.4. Concentration of the Substrate pAPP from Adhesion Cells
3.5. Comparative Study of ALP Activity
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cell Line | Concentration (×103 Cells/mL) | Media | Supplements |
---|---|---|---|
Balb/c 3t3 | 40 | DMEM | 10% NBCS |
A549 | 40 | DMEM | 10% FBS |
MCF-7 | 40 | MEME | 10% FBS |
Ht-29 | 80 | McCoy’s 5A | 10% FBS |
Scan Rate | a Emid vs. Ag/AgCl | b ∆Ep (mV) | ||||
---|---|---|---|---|---|---|
0 min | 10 min | 20 min | 0 min | 10 min | 20 min | |
5 | 0.2285 | 0.0768 | 0.198 | 175 | 146.4 | 76 |
10 | 0.2465 | 0.08145 | 0.194 | 207 | 143.1 | 68 |
20 | 0.261 | 0.09155 | 0.192 | 230 | 144.9 | 68 |
50 | 0.2715 | 0.1163 | 0.192 | 247 | 137.4 | 64 |
70 | 0.277 | 0.1225 | 0.191 | 260 | 151 | 62 |
100 | 0.282 | 0.1305 | 0.191 | 270 | 153 | 62 |
150 | 0.294 | 0.135 | 0.194 | 288 | 162 | 64 |
200 | 0.298 | 0.1585 | 0.193 | 300 | 163 | 58 |
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Balbaied, T.; Hogan, A.; Moore, E. Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells. Biosensors 2020, 10, 95. https://doi.org/10.3390/bios10080095
Balbaied T, Hogan A, Moore E. Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells. Biosensors. 2020; 10(8):95. https://doi.org/10.3390/bios10080095
Chicago/Turabian StyleBalbaied, Thanih, Anna Hogan, and Eric Moore. 2020. "Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells" Biosensors 10, no. 8: 95. https://doi.org/10.3390/bios10080095
APA StyleBalbaied, T., Hogan, A., & Moore, E. (2020). Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells. Biosensors, 10(8), 95. https://doi.org/10.3390/bios10080095