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Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells

Sensing & Separation Group, School of Chemistry and Life Science Interface, University College Cork, Tyndall National Institute, T12R5CP Cork, Ireland
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Biosensors 2020, 10(8), 95; https://doi.org/10.3390/bios10080095
Received: 30 June 2020 / Revised: 3 August 2020 / Accepted: 4 August 2020 / Published: 11 August 2020
(This article belongs to the Section Biosensor and Bioelectronic Devices)
Alkaline phosphatase (ALP) is one of the main biomarkers that is clinically detected in bone and liver disorders using optical assays. The electrochemical principle is important because point-of-care testing is increasing dramatically and absorbance techniques hardly compete with the medical revolution that is occurring. The detection of ALP using electrochemical detection is contributing to the integration systems field, and hence enhancing the detection of biological targets for pharmaceutical research and design systems. Moreover, in vitro electrochemical measurements use cost effective materials and simple techniques. Graphite screen-printed electrodes and linear sweep voltammetry were used to optimize the electrochemistry of the enzymatic product p-aminophenol using the enzyme kinetic assay. ALP release from embryonic and cancer cells was determined from adhesion cell culture. Additionally, capillary electrophoresis and colorimetric methods were applied for comparison assays. The resulting assays showed a dynamic range of ALP ranging from 1.5 to 1500 U/L, and limit of detection of 0.043 U/L. This was achieved by using 70 μL of the sample and an incubation time of 10 min at an optimal substrate concentration of 9.6 mM of p-aminophenol phosphate. A significant difference (p < 0.05) was measured between the absorbance assays. This paper demonstrates the advantages of the electrochemical assay for ALP release from cells, which is in line with recent trends in gene expression systems using microelectrode array technologies and devices for monitoring electrophysiological activity. View Full-Text
Keywords: electrochemistry; linear sweep voltammetry; alkaline phosphatase; capillary electrophoresis; colorimetry; cancer cells; embryonic cells electrochemistry; linear sweep voltammetry; alkaline phosphatase; capillary electrophoresis; colorimetry; cancer cells; embryonic cells
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MDPI and ACS Style

Balbaied, T.; Hogan, A.; Moore, E. Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells. Biosensors 2020, 10, 95. https://doi.org/10.3390/bios10080095

AMA Style

Balbaied T, Hogan A, Moore E. Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells. Biosensors. 2020; 10(8):95. https://doi.org/10.3390/bios10080095

Chicago/Turabian Style

Balbaied, Thanih; Hogan, Anna; Moore, Eric. 2020. "Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells" Biosensors 10, no. 8: 95. https://doi.org/10.3390/bios10080095

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