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Review

Review of Transducer Principles for Label-Free Biomolecular Interaction Analysis

1
Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland
2
Siemens Technology Accelerator GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany
*
Author to whom correspondence should be addressed.
Biosensors 2011, 1(3), 70-92; https://doi.org/10.3390/bios1030070
Received: 19 May 2011 / Revised: 18 June 2011 / Accepted: 29 June 2011 / Published: 1 July 2011
Label-free biomolecular interaction analysis is an important technique to study the chemical binding between e.g., protein and protein or protein and small molecule in real-time. The parameters obtained with this technique, such as the affinity, are important for drug development. While the surface plasmon resonance (SPR) instruments are most widely used, new types of sensors are emerging. These developments are generally driven by the need for higher throughput, lower sample consumption or by the need of complimentary information to the SPR data. This review aims to give an overview about a wide range of sensor transducers, the working principles and the peculiarities of each technology, e.g., concerning the set-up, sensitivity, sensor size or required sample volume. Starting from optical technologies like the SPR and waveguide based sensors, acoustic sensors like the quartz crystal microbalance (QCM) and the film bulk acoustic resonator (FBAR), calorimetric and electrochemical sensors are covered. Technologies long established in the market are presented together with those newly commercially available and with technologies in the early development stage. Finally, the commercially available instruments are summarized together with their sensitivity and the number of sensors usable in parallel and an outlook for potential future developments is given. View Full-Text
Keywords: biomolecular interaction analysis; BIA; sensor; transducer; drug discovery; drug development biomolecular interaction analysis; BIA; sensor; transducer; drug discovery; drug development
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MDPI and ACS Style

Nirschl, M.; Reuter, F.; Vörös, J. Review of Transducer Principles for Label-Free Biomolecular Interaction Analysis. Biosensors 2011, 1, 70-92. https://doi.org/10.3390/bios1030070

AMA Style

Nirschl M, Reuter F, Vörös J. Review of Transducer Principles for Label-Free Biomolecular Interaction Analysis. Biosensors. 2011; 1(3):70-92. https://doi.org/10.3390/bios1030070

Chicago/Turabian Style

Nirschl, Martin, Florian Reuter, and Janos Vörös. 2011. "Review of Transducer Principles for Label-Free Biomolecular Interaction Analysis" Biosensors 1, no. 3: 70-92. https://doi.org/10.3390/bios1030070

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