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Characterizing the Cellular Response to Nitrogen-Doped Carbon Nanocups

Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, 41470 Gebze/Kocaeli, Turkey
School of Medicine, Koc University, 34450 Sarıyer, Turkey
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(6), 887;
Received: 23 May 2019 / Revised: 6 June 2019 / Accepted: 7 June 2019 / Published: 16 June 2019
PDF [2306 KB, uploaded 16 June 2019]
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Carbon nanomaterials, specifically, carbon nanotubes (CNTs) have many potential applications in biology and medicine. Currently, this material has not reached its full potential for application due to the potential toxicity to mammalian cells, and the incomplete understanding of how CNTs interface with cells. The chemical composition and structural features of CNTs have been shown to directly affect their biological compatibility. The incorporation of nitrogen dopants to the graphitic lattice of CNTs results in a unique cup shaped morphology and minimal cytotoxicity in comparison to its undoped counterpart. In this study, we investigate how uniquely shaped nitrogen-doped carbon nanocups (NCNCs) interface with HeLa cells, a cervical cancer epithelial cultured cell line, and RPE-1 cells, an immortalized cultured epithelial cell line. We determined that NCNCs do not elicit a cytotoxic response in cells, and that they are uptaken via endocytosis. We have conjugated fluorescently tagged antibodies to NCNCs and shown that the protein-conjugated material is also capable of entering cells. This primes NCNCs to be a good candidate for subsequent protein modifications and applications in biological systems. View Full-Text
Keywords: nanocups; nanotubes; biocompatibility nanocups; nanotubes; biocompatibility

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Griffith, A.S.; Zhang, T.D.; Burkert, S.C.; Adiguzel, Z.; Acilan, C.; Star, A.; Saunders, W.S. Characterizing the Cellular Response to Nitrogen-Doped Carbon Nanocups. Nanomaterials 2019, 9, 887.

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