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Colorimetric Method for Sensitive Detection of Microcystin-LR Using Surface Copper Nanoparticles of Polydopamine Nanosphere as Turn-On Probe

1
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
2
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China
*
Authors to whom correspondence should be addressed.
Nanomaterials 2019, 9(3), 332; https://doi.org/10.3390/nano9030332
Received: 6 February 2019 / Revised: 17 February 2019 / Accepted: 19 February 2019 / Published: 2 March 2019
A novel, facile sensor was further developed for microcystin-LR (MC-LR) determination by visible spectroscopy. Antibody-functionalized SiO2-coated magnetic nanoparticles (Fe3O4@SiO2) and aptamer-functionalized polydopamine nanospheres decorated with Cu nanoparticles (PDA/CuNPs) recognized specific sites in MC-LR and then the sandwich-type composites were separated magnetically. The Cu in the separated composites was converted to Cu2+ ions in solution and turn-on visible absorption was achieved after reaction with bis(cyclohexanone)oxaldihydrazone (BCO) (λmax = 600 nm). There was a quantitative relationship between the spectral intensity and MC-LR concentration. In addition, under the optimum conditions, the sensor turns out to be a linear relationship from 0.05 to 25 nM, with a limit of detection of 0.05 nM (0.05 μg/L) (S/N = 3) for MC-LR. The sensitivity was dependent on the low background absorption from the off-to-on spectrum and label amplification by the polydopamine (PDA) surface. The sensor had high selectivity, which shows the importance of dual-site recognition by the aptamer and antibody and the highly specific color formed by BCO with Cu2+. The bioassay was complete within 150 min, which enabled quick determination. The sensor was successfully used with real spiked samples. These results suggest it has potential applications in visible detection and could be used to detect other microcystin analogs. View Full-Text
Keywords: turn-on visible absorption; dual-site recognition; magnetic separation; microcystin-LR; colorimetric sensor turn-on visible absorption; dual-site recognition; magnetic separation; microcystin-LR; colorimetric sensor
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MDPI and ACS Style

Tang, X.; Yin, Z.; Lei, X.; Zeng, Y.; Zhang, Z.; Lu, Y.; Zhou, G.; Li, L.; Wu, X. Colorimetric Method for Sensitive Detection of Microcystin-LR Using Surface Copper Nanoparticles of Polydopamine Nanosphere as Turn-On Probe. Nanomaterials 2019, 9, 332. https://doi.org/10.3390/nano9030332

AMA Style

Tang X, Yin Z, Lei X, Zeng Y, Zhang Z, Lu Y, Zhou G, Li L, Wu X. Colorimetric Method for Sensitive Detection of Microcystin-LR Using Surface Copper Nanoparticles of Polydopamine Nanosphere as Turn-On Probe. Nanomaterials. 2019; 9(3):332. https://doi.org/10.3390/nano9030332

Chicago/Turabian Style

Tang, Xiaodi; Yin, Zhengzhi; Lei, Xiaoling; Zeng, Yanbo; Zhang, Zulei; Lu, Yixia; Zhou, Guobao; Li, Lei; Wu, Xiaohua. 2019. "Colorimetric Method for Sensitive Detection of Microcystin-LR Using Surface Copper Nanoparticles of Polydopamine Nanosphere as Turn-On Probe" Nanomaterials 9, no. 3: 332. https://doi.org/10.3390/nano9030332

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