Next Article in Journal
Photothermally Controlled Methotrexate Release System Using β-Cyclodextrin and Gold Nanoparticles
Next Article in Special Issue
Vectors for Glioblastoma Gene Therapy: Viral & Non-Viral Delivery Strategies
Previous Article in Journal
Hierarchical Ta-Doped TiO2 Nanorod Arrays with Improved Charge Separation for Photoelectrochemical Water Oxidation under FTO Side Illumination
Open AccessArticle

Fluorogenic RNA Aptamers: A Nano-platform for Fabrication of Simple and Combinatorial Logic Gates

Department of Chemistry, Ball State University, Muncie, IN 47304, USA
Author to whom correspondence should be addressed.
Nanomaterials 2018, 8(12), 984;
Received: 25 October 2018 / Revised: 16 November 2018 / Accepted: 20 November 2018 / Published: 28 November 2018
RNA aptamers that bind non-fluorescent dyes and activate their fluorescence are highly sensitive, nonperturbing, and convenient probes in the field of synthetic biology. These RNA molecules, referred to as light-up aptamers, operate as molecular nanoswitches that alter folding and fluorescence function in response to ligand binding, which is important in biosensing and molecular computing. Herein, we demonstrate a conceptually new generation of smart RNA nano-devices based on malachite green (MG)-binding RNA aptamer, which fluorescence output controlled by addition of short DNA oligonucleotides inputs. Four types of RNA switches possessing AND, OR, NAND, and NOR Boolean logic functions were created in modular form, allowing MG dye binding affinity to be changed by altering 3D conformation of the RNA aptamer. It is essential to develop higher-level logic circuits for the production of multi-task nanodevices for data processing, typically requiring combinatorial logic gates. Therefore, we further designed and synthetized higher-level half adder logic circuit by “in parallel” integration of two logic gates XOR and AND within a single RNA nanoparticle. The design utilizes fluorescence emissions from two different RNA aptamers: MG-binding RNA aptamer (AND gate) and Broccoli RNA aptamer that binds DFHBI dye (XOR gate). All computationally designed RNA devices were synthesized and experimentally tested in vitro. The ability to design smart nanodevices based on RNA binding aptamers offers a new route to engineer “label-free” ligand-sensing regulatory circuits, nucleic acid detection systems, and gene control elements. View Full-Text
Keywords: logic gates; nucleic acid computing; RNA aptamers; RNA nanotechnology logic gates; nucleic acid computing; RNA aptamers; RNA nanotechnology
Show Figures

Graphical abstract

MDPI and ACS Style

Goldsworthy, V.; LaForce, G.; Abels, S.; Khisamutdinov, E.F. Fluorogenic RNA Aptamers: A Nano-platform for Fabrication of Simple and Combinatorial Logic Gates. Nanomaterials 2018, 8, 984.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop