A New Label-Free Technique for Analysing Evaporation Induced Self-Assembly of Viral Nanoparticles Based on Enhanced Dark-Field Optical Imaging
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteriophage Propagation and Purification
2.2. Experimental Setup: Enhanced Dark-Field Microscopy and Monitoring of Evaporation Process
2.3. Particle Tracking and Image Analysis
3. Results and Discussion
3.1. Analysing the Bacteriophage Motion and Its Influence on Phage Assembly onto the Glass Coverslip
3.2. Analysing Fluid Flow through Monitoring Drying Process and Final Dried Patterns (Macroscopic Observations)
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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T4 Phage Suspensions | Diffusion Coefficient (cm2/s) |
---|---|
T4 phage suspension in water at 23 °C (108 to 109 pfu/mL) [52] | 4 × 10−8–8 × 10−8 |
T4 phage suspension in SM buffer (109 pfu/mL) | 4.8 × 10−8 |
Concentrated T4 phage suspension in water (1011 pfu/mL) | 5.6 × 10−10 |
Diluted T4 phage suspension in water (109 pfu/mL) | 3.6 × 10−8 |
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Ghaeli, I.; Hosseinidoust, Z.; Zolfagharnasab, H.; Jorge Monteiro, F. A New Label-Free Technique for Analysing Evaporation Induced Self-Assembly of Viral Nanoparticles Based on Enhanced Dark-Field Optical Imaging. Nanomaterials 2018, 8, 1. https://doi.org/10.3390/nano8010001
Ghaeli I, Hosseinidoust Z, Zolfagharnasab H, Jorge Monteiro F. A New Label-Free Technique for Analysing Evaporation Induced Self-Assembly of Viral Nanoparticles Based on Enhanced Dark-Field Optical Imaging. Nanomaterials. 2018; 8(1):1. https://doi.org/10.3390/nano8010001
Chicago/Turabian StyleGhaeli, Ima, Zeinab Hosseinidoust, Hooshiar Zolfagharnasab, and Fernando Jorge Monteiro. 2018. "A New Label-Free Technique for Analysing Evaporation Induced Self-Assembly of Viral Nanoparticles Based on Enhanced Dark-Field Optical Imaging" Nanomaterials 8, no. 1: 1. https://doi.org/10.3390/nano8010001