The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production and Characterization of Green Chemistry Silver Nanoparticles
2.1.1. UV-Vis Spectroscopy
2.1.2. Dynamic Light Scattering
2.1.3. Transmission Electron Microscopy
2.1.4. Zeta Potential
2.2. Efficacy Evaluation Assays
2.2.1. Bacterial Strains and Growth Conditions
2.2.2. Broth Microdilution Method
2.3. Scanning Electron Microscopy
2.4. Data Analysis
3. Results
3.1. Characterization of NP1 and NP2
3.1.1. UV-Vis Spectroscopy
3.1.2. Size and Shape of NPs Characterized by Dynamic Light Scattering and Transmission Electron Microscopy
3.1.3. Surface Charge of NPs Measured by Zeta Potential
3.2. Kinetics of Growth of Bacterial Pathogens under NP Antibiosis
3.3. Dose Response Effect of NP1 and NP2 against Bacterial Pathogens
3.4. Morphology of E. coli Cells under NP1 and NP2 Antibiosis
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Species | MIC (μg/mL) | MIC50 (μg/mL) | Range Concentrations (≥) (μg/mL) | MBC (μg/mL) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
NP1 | NP2 | NP1 | NP2 | NP1 12 h | NP2 12 h | NP1 24 h | NP2 24 h | NP1 | NP2 | |
S. epidermidis | 15 | 15 | 6.6 | 6.3 | - | - | 15 | 15 | 15 | 15 |
B. subtilis | 3 | 3 | 2 | 1.8 | 3 | 3 | 3 | 3 | 3 | 3 |
E. coli | 15 | 15 | 9 | 9.9 | 3 | 3 | 15 | 15 | 15 | 15 |
P. aeruginosa | 15 | 15 | 3.6 | 3.6 | - | - | 15 | 15 | 15 | 15 |
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Skandalis, N.; Dimopoulou, A.; Georgopoulou, A.; Gallios, N.; Papadopoulos, D.; Tsipas, D.; Theologidis, I.; Michailidis, N.; Chatzinikolaidou, M. The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy. Nanomaterials 2017, 7, 178. https://doi.org/10.3390/nano7070178
Skandalis N, Dimopoulou A, Georgopoulou A, Gallios N, Papadopoulos D, Tsipas D, Theologidis I, Michailidis N, Chatzinikolaidou M. The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy. Nanomaterials. 2017; 7(7):178. https://doi.org/10.3390/nano7070178
Chicago/Turabian StyleSkandalis, Nicholas, Anastasia Dimopoulou, Anthie Georgopoulou, Nikolaos Gallios, Dimitrios Papadopoulos, Dimitrios Tsipas, Ioannis Theologidis, Nikolaos Michailidis, and Maria Chatzinikolaidou. 2017. "The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy" Nanomaterials 7, no. 7: 178. https://doi.org/10.3390/nano7070178