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Open AccessArticle

Synthesis and Application of Amine Functionalized Iron Oxide Nanoparticles on Menaquinone-7 Fermentation: A Step towards Process Intensification

1
Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa 74615, Iran
2
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 71348, Iran
3
School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton 3240, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editor: Yurii Gun'ko
Nanomaterials 2016, 6(1), 1; https://doi.org/10.3390/nano6010001
Received: 17 November 2015 / Revised: 19 December 2015 / Accepted: 21 December 2015 / Published: 25 December 2015
Industrial production of menaquione-7 by Bacillus subtilis natto is associated with major drawbacks. To address the current challenges in menaquione-7 fermentation, studying the effect of magnetic nanoparticles on the bacterial cells can open up a new domain for intensified menqainone-7 process. This article introduces the new concept of production and application of l-lysine coated iron oxide nanoparticles (l[email protected]) as a novel tool for menaquinone-7 biosynthesis. l[email protected] with the average size of 7 nm were successfully fabricated and were examined in a fermentation process of l[email protected] decorated Bacillus subtilis natto. Based on the results, higher menaquinone-7 specific yield was observed for l[email protected] decorated bacterial cells as compared to untreated bacteria. In addition, more than 92% removal efficacy was achieved by using integrated magnetic separation process. The present study demonstrates that l[email protected] can be successfully applied during a fermentation of menaquinone-7 without any negative consequences on the culture conditions. This study provides a novel biotechnological application for IONs and their future role in bioprocess intensification. View Full-Text
Keywords: immobilization; downstream processing; magnetic nanoparticles; MK-7; bioseparations; fermentation immobilization; downstream processing; magnetic nanoparticles; MK-7; bioseparations; fermentation
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Ebrahiminezhad, A.; Varma, V.; Yang, S.; Ghasemi, Y.; Berenjian, A. Synthesis and Application of Amine Functionalized Iron Oxide Nanoparticles on Menaquinone-7 Fermentation: A Step towards Process Intensification. Nanomaterials 2016, 6, 1.

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