Next Article in Journal
Microbial Decontamination and Antibacterial Activity of Nanostructured Titanium Dental Implants: A Narrative Review
Previous Article in Journal
Highly Sensitive and Stretchable c-MWCNTs/PPy Embedded Multidirectional Strain Sensor Based on Double Elastic Fabric for Human Motion Detection
Previous Article in Special Issue
A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs
 
 
Article

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

1
Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, China
2
Materials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
*
Author to whom correspondence should be addressed.
Academic Editors: Costantino Del Gaudio, Takuya Kitaoka, Silvia Baiguera and Lucy Di Silvio
Nanomaterials 2021, 11(9), 2334; https://doi.org/10.3390/nano11092334
Received: 28 June 2021 / Revised: 3 September 2021 / Accepted: 6 September 2021 / Published: 8 September 2021
(This article belongs to the Special Issue Moving toward Biomimetic Tissue Engineered Scaffolds)
In this study, we designed and synthetized artificial vascular scaffolds based on nanofibers of collagen functionalized with hyaluronic acid (HA) in order to direct the phenotypic shape, proliferation, and complete endothelization of mouse primary aortic endothelial cells (PAECs). Layered tubular HA/collagen nanofibers were prepared using electrospinning and crosslinking process. The obtained scaffold is composed of a thin inner layer and a thick outer layer that structurally mimic the layer the intima and media layers of the native blood vessels, respectively. Compared with the pure tubular collagen nanofibers, the surface of HA functionalized collagen nanofibers has higher anisotropic wettability and mechanical flexibility. HA/collagen nanofibers can significantly promote the elongation, proliferation and phenotypic shape expression of PAECs. In vitro co-culture of mouse PAECs and their corresponding smooth muscle cells (SMCs) showed that the luminal endothelialization governs the biophysical integrity of the newly formed extracellular matrix (e.g., collagen and elastin fibers) and structural remodeling of SMCs. Furthermore, in vitro hemocompatibility assays indicated that HA/collagen nanofibers have no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular implants. View Full-Text
Keywords: collagen; hyaluronic acid; vascular endothelial cells; nanofibers; endothelization collagen; hyaluronic acid; vascular endothelial cells; nanofibers; endothelization
Show Figures

Figure 1

MDPI and ACS Style

Niu, Y.; Galluzzi, M. Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization. Nanomaterials 2021, 11, 2334. https://doi.org/10.3390/nano11092334

AMA Style

Niu Y, Galluzzi M. Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization. Nanomaterials. 2021; 11(9):2334. https://doi.org/10.3390/nano11092334

Chicago/Turabian Style

Niu, Yuqing, and Massimiliano Galluzzi. 2021. "Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization" Nanomaterials 11, no. 9: 2334. https://doi.org/10.3390/nano11092334

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop