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Article

Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

1
Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany
2
School of Analytical Sciences Adlershof, Humboldt-Universität zu Berlin, Albert-Einstein-Str. 5-9, 12489 Berlin, Germany
3
Department X-ray Microscopy, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Kerstin Leopold
Nanomaterials 2021, 11(5), 1183; https://doi.org/10.3390/nano11051183
Received: 8 April 2021 / Revised: 26 April 2021 / Accepted: 27 April 2021 / Published: 30 April 2021
(This article belongs to the Special Issue Development of Nanomaterials for Applications in Trace Analysis)
Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics. View Full-Text
Keywords: gold nanostars; SERS; nanoparticle uptake; nanotomography; endocytosis; protein corona; HCT-116; J774; 3T3 gold nanostars; SERS; nanoparticle uptake; nanotomography; endocytosis; protein corona; HCT-116; J774; 3T3
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MDPI and ACS Style

Spedalieri, C.; Szekeres, G.P.; Werner, S.; Guttmann, P.; Kneipp, J. Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars. Nanomaterials 2021, 11, 1183. https://doi.org/10.3390/nano11051183

AMA Style

Spedalieri C, Szekeres GP, Werner S, Guttmann P, Kneipp J. Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars. Nanomaterials. 2021; 11(5):1183. https://doi.org/10.3390/nano11051183

Chicago/Turabian Style

Spedalieri, Cecilia, Gergo P. Szekeres, Stephan Werner, Peter Guttmann, and Janina Kneipp. 2021. "Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars" Nanomaterials 11, no. 5: 1183. https://doi.org/10.3390/nano11051183

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