Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Biomimetic Magnetic Nanoparticles Synthesis
2.3. PLGA Empty Nanoparticles Synthesis
2.4. PLGA Encapsulation of BMNPs and TAT Peptide Functionalization
2.5. Dynamic Light Scattering and Zeta Potential
2.6. Atomic Force Microscopy
2.7. Hysteresis Cycle at 5 and 300 K and Magnetic Hyperthermia
2.8. Transmission Electron Microscopy Analysis
2.9. Quantitative Analysis of Nanoparticle Internalization
2.10. Cell Proliferation Assay
2.11. In Vitro Cytotoxicity of the Nanoformulation
3. Results and Discussion
3.1. Synthesis and Characterization of Biomimetic Magnetic Nanoparticles (BMNPs) and the Nanoformulations of PLGA (BMNPs) and TAT-PLGA(BMNPs)
3.2. Magnetic Saturation and Magnetic Hyperthermia of the Nanoformulations
3.3. Enhanced Cellular Uptake of the Nanoformulations versus BMNPs
3.4. Cytocompatibility of PLGA(BMNPs)
3.5. Cytotoxicity of PLGA(BMNPs)
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Vurro, F.; Jabalera, Y.; Mannucci, S.; Glorani, G.; Sola-Leyva, A.; Gerosa, M.; Romeo, A.; Romanelli, M.G.; Malatesta, M.; Calderan, L.; et al. Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles. Nanomaterials 2021, 11, 766. https://doi.org/10.3390/nano11030766
Vurro F, Jabalera Y, Mannucci S, Glorani G, Sola-Leyva A, Gerosa M, Romeo A, Romanelli MG, Malatesta M, Calderan L, et al. Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles. Nanomaterials. 2021; 11(3):766. https://doi.org/10.3390/nano11030766
Chicago/Turabian StyleVurro, Federica, Ylenia Jabalera, Silvia Mannucci, Giulia Glorani, Alberto Sola-Leyva, Marco Gerosa, Alessandro Romeo, Maria Grazia Romanelli, Manuela Malatesta, Laura Calderan, and et al. 2021. "Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles" Nanomaterials 11, no. 3: 766. https://doi.org/10.3390/nano11030766